Quantitative analysis of redox-sensitive proteome with DIGE and ICAT

Cexiong Fu, Jun Hu, Tong Liu, Tetsuro Ago, Junichi Sadoshima, Hong Li

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)

Abstract

Oxidative modifications of protein thiols are important mechanisms for regulating protein functions. The present study aimed to compare the relative effectiveness of two thiol-specific quantitative proteomic techniques, difference gel electrophoresis (DIGE) and isotope coded affinity tag (ICAT), for the discovery of redox-sensitive proteins in heart tissues. We found that these two methods were largely complementary; each could be used to reveal a set of unique redox-sensitive proteins. Some of these proteins are low-abundant signaling proteins and membrane proteins. From DIGE analysis, we found that both NF-κ:B-repressing protein and epoxide hydrolase were sensitive to H 2O 2 oxidation. In ICAT analysis, we found that specific cysteines within sacroplasmic endoplamic reticulum calcium ATPase 2 and voltage-dependent anion-selective channel protein 1 were sensitive to H 2O 2 oxidation. From these analyses, we conclude that both methods should be employed for proteome-wide studies, to maximize the possibility of identifying proteins containing redox-sensitive cysteinyl thiols in complex biological systems.

Original languageEnglish
Pages (from-to)3789-3802
Number of pages14
JournalJournal of Proteome Research
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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