Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex

Laurent Gapin, Yoshinori Fukui, Jean Kanellopoulos, Tetsuro Sano, Armanda Casrouge, Vanessa Malier, Emmanuel Beaudoing, Daniel Gautheret, Jean Michel Claverie, Takehiko Sasazuki, Philippe Kourilsky

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

The positive selection of CD4+ T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide-MHC class II complex positively select significant numbers of diverse CD4+ T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide-MHC class II complex positively selects at least 105 different Vβ rearrangements. Our data yield a first evaluation oF the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68-I-Ab complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide.

Original languageEnglish
Pages (from-to)1871-1883
Number of pages13
JournalJournal of Experimental Medicine
Volume187
Issue number11
DOIs
Publication statusPublished - Jun 1 1998

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex'. Together they form a unique fingerprint.

Cite this