Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex

Laurent Gapin, Yoshinori Fukui, Jean Kanellopoulos, Tetsuro Sano, Armanda Casrouge, Vanessa Malier, Emmanuel Beaudoing, Daniel Gautheret, Jean Michel Claverie, Takehiko Sasazuki, Philippe Kourilsky

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The positive selection of CD4+ T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide-MHC class II complex positively select significant numbers of diverse CD4+ T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide-MHC class II complex positively selects at least 105 different Vβ rearrangements. Our data yield a first evaluation oF the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68-I-Ab complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide.

Original languageEnglish
Pages (from-to)1871-1883
Number of pages13
JournalJournal of Experimental Medicine
Volume187
Issue number11
DOIs
Publication statusPublished - Jun 1 1998

Fingerprint

Complementarity Determining Regions
Major Histocompatibility Complex
T-Lymphocytes
Peptides
T-Cell Antigen Receptor
Thymus Gland
T-Cell Antigen Receptor Specificity
Transgenic Mice
Amino Acids

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex. / Gapin, Laurent; Fukui, Yoshinori; Kanellopoulos, Jean; Sano, Tetsuro; Casrouge, Armanda; Malier, Vanessa; Beaudoing, Emmanuel; Gautheret, Daniel; Claverie, Jean Michel; Sasazuki, Takehiko; Kourilsky, Philippe.

In: Journal of Experimental Medicine, Vol. 187, No. 11, 01.06.1998, p. 1871-1883.

Research output: Contribution to journalArticle

Gapin, L, Fukui, Y, Kanellopoulos, J, Sano, T, Casrouge, A, Malier, V, Beaudoing, E, Gautheret, D, Claverie, JM, Sasazuki, T & Kourilsky, P 1998, 'Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex', Journal of Experimental Medicine, vol. 187, no. 11, pp. 1871-1883. https://doi.org/10.1084/jem.187.11.1871
Gapin, Laurent ; Fukui, Yoshinori ; Kanellopoulos, Jean ; Sano, Tetsuro ; Casrouge, Armanda ; Malier, Vanessa ; Beaudoing, Emmanuel ; Gautheret, Daniel ; Claverie, Jean Michel ; Sasazuki, Takehiko ; Kourilsky, Philippe. / Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex. In: Journal of Experimental Medicine. 1998 ; Vol. 187, No. 11. pp. 1871-1883.
@article{664c24e833ee4e2b891541241951ba5e,
title = "Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex",
abstract = "The positive selection of CD4+ T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide-MHC class II complex positively select significant numbers of diverse CD4+ T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide-MHC class II complex positively selects at least 105 different Vβ rearrangements. Our data yield a first evaluation oF the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68-I-Ab complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide.",
author = "Laurent Gapin and Yoshinori Fukui and Jean Kanellopoulos and Tetsuro Sano and Armanda Casrouge and Vanessa Malier and Emmanuel Beaudoing and Daniel Gautheret and Claverie, {Jean Michel} and Takehiko Sasazuki and Philippe Kourilsky",
year = "1998",
month = "6",
day = "1",
doi = "10.1084/jem.187.11.1871",
language = "English",
volume = "187",
pages = "1871--1883",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "11",

}

TY - JOUR

T1 - Quantitative analysis of the T cell repertoire selected by a single peptide-major histocompatibility complex

AU - Gapin, Laurent

AU - Fukui, Yoshinori

AU - Kanellopoulos, Jean

AU - Sano, Tetsuro

AU - Casrouge, Armanda

AU - Malier, Vanessa

AU - Beaudoing, Emmanuel

AU - Gautheret, Daniel

AU - Claverie, Jean Michel

AU - Sasazuki, Takehiko

AU - Kourilsky, Philippe

PY - 1998/6/1

Y1 - 1998/6/1

N2 - The positive selection of CD4+ T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide-MHC class II complex positively select significant numbers of diverse CD4+ T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide-MHC class II complex positively selects at least 105 different Vβ rearrangements. Our data yield a first evaluation oF the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68-I-Ab complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide.

AB - The positive selection of CD4+ T cells requires the expression of major histocompatibility complex (MHC) class II molecules in the thymus, but the role of self-peptides complexed to class II molecules is still a matter of debate. Recently, it was observed that transgenic mice expressing a single peptide-MHC class II complex positively select significant numbers of diverse CD4+ T cells in the thymus. However, the number of selected T cell specificities has not been evaluated so far. Here, we have sequenced 700 junctional complementarity determining regions 3 (CDR3) from T cell receptors (TCRs) carrying Vβ11-Jβ1.1 or Vβ12-Jβ1.1 rearrangements. We found that a single peptide-MHC class II complex positively selects at least 105 different Vβ rearrangements. Our data yield a first evaluation oF the size of the T cell repertoire. In addition, they provide evidence that the single Eα52-68-I-Ab complex skews the amino acid frequency in the TCR CDR3 loop of positively selected T cells. A detailed analysis of CDR3 sequences indicates that a fraction of the β chain repertoire bears the imprint of the selecting self-peptide.

UR - http://www.scopus.com/inward/record.url?scp=17644440779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17644440779&partnerID=8YFLogxK

U2 - 10.1084/jem.187.11.1871

DO - 10.1084/jem.187.11.1871

M3 - Article

C2 - 9607927

AN - SCOPUS:17644440779

VL - 187

SP - 1871

EP - 1883

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 11

ER -