TY - JOUR
T1 - Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source
AU - Okuno, Masanari
AU - Kano, Hideaki
AU - Leproux, Philippe
AU - Couderc, Vincent
AU - Hamaguchi, Hiro O.
N1 - Funding Information:
This work is supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support by, Grand-Aid for Scientific Research on Priority Areas “Molecular Science for Supra Functional Systems” [477] from MEXT, and the Global COE Program for “Chemistry Innovation”. The authors gratefully acknowledge J. Ukon, HORIBA, Ltd. for assisting a fruitful collaboration between Japanese and French labs.
PY - 2012/9
Y1 - 2012/9
N2 - We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm-1) and an ultrabroadband spectral coverage (>2500 cm-1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.
AB - We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm-1) and an ultrabroadband spectral coverage (>2500 cm-1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.
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U2 - 10.1016/j.yofte.2012.05.006
DO - 10.1016/j.yofte.2012.05.006
M3 - Article
AN - SCOPUS:84867210861
VL - 18
SP - 388
EP - 393
JO - Optical Fiber Technology
JF - Optical Fiber Technology
SN - 1068-5200
IS - 5
ER -