Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source

Masanari Okuno; Hideaki Kano; Philippe Leproux; Vincent Couderc; Hiro O. Hamaguchi

doi:10.1016/j.yofte.2012.05.006

Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source

Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, Hiro O. Hamaguchi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm^-1) and an ultrabroadband spectral coverage (>2500 cm^-1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.

Original language	English
Pages (from-to)	388-393
Number of pages	6
Journal	Optical Fiber Technology
Volume	18
Issue number	5
DOIs	https://doi.org/10.1016/j.yofte.2012.05.006
Publication status	Published - Sep 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Control and Systems Engineering
Atomic and Molecular Physics, and Optics
Instrumentation
Electrical and Electronic Engineering

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10.1016/j.yofte.2012.05.006

Cite this

@article{867f1d2f02504fa2ae66405761536d7c,

title = "Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source",

abstract = "We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm-1) and an ultrabroadband spectral coverage (>2500 cm-1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.",

author = "Masanari Okuno and Hideaki Kano and Philippe Leproux and Vincent Couderc and Hamaguchi, {Hiro O.}",

note = "Funding Information: This work is supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support by, Grand-Aid for Scientific Research on Priority Areas “Molecular Science for Supra Functional Systems” [477] from MEXT, and the Global COE Program for “Chemistry Innovation”. The authors gratefully acknowledge J. Ukon, HORIBA, Ltd. for assisting a fruitful collaboration between Japanese and French labs.",

year = "2012",

month = sep,

doi = "10.1016/j.yofte.2012.05.006",

language = "English",

volume = "18",

pages = "388--393",

journal = "Optical Fiber Technology",

issn = "1068-5200",

publisher = "Academic Press Inc.",

number = "5",

}

TY - JOUR

T1 - Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source

AU - Okuno, Masanari

AU - Kano, Hideaki

AU - Leproux, Philippe

AU - Couderc, Vincent

AU - Hamaguchi, Hiro O.

N1 - Funding Information: This work is supported by the SENTAN project (Program-S) of the Japan Science and Technology Agency (JST). H. Kano gratefully acknowledges financial support by, Grand-Aid for Scientific Research on Priority Areas “Molecular Science for Supra Functional Systems” [477] from MEXT, and the Global COE Program for “Chemistry Innovation”. The authors gratefully acknowledge J. Ukon, HORIBA, Ltd. for assisting a fruitful collaboration between Japanese and French labs.

PY - 2012/9

Y1 - 2012/9

N2 - We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm-1) and an ultrabroadband spectral coverage (>2500 cm-1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.

AB - We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm-1) and an ultrabroadband spectral coverage (>2500 cm-1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.

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U2 - 10.1016/j.yofte.2012.05.006

DO - 10.1016/j.yofte.2012.05.006

M3 - Article

AN - SCOPUS:84867210861

VL - 18

SP - 388

EP - 393

JO - Optical Fiber Technology

JF - Optical Fiber Technology

SN - 1068-5200

IS - 5

ER -

Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source

Abstract

All Science Journal Classification (ASJC) codes

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