Quantitative determination of amino acids in functional foods by microchip electrophoresis

Hiroko Ueno, Jun Wang, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Microchip electrophoresis (MCE), a first-generation micrototal analysis system, has emerged during the miniaturization phase of food analysis. Based on the micellar electrokinetic chromatography mode, a simple and fast MCE method with light emitting diode-induced fluorescence detection was developed for quantitative analysis of amino acids in three different kinds of functional foods, viz. sports beverages, jelly-form beverages, and tablet-form functional foods. In contrast to the glass microchip, we improved the separation of amino acids on a poly(methyl methacrylate) (PMMA) chip by addition of cationic starch derivatives. 4-Fluoro-7-nitro-2,1,3-benzoxadiazole, which has a short labeling time for amino acids, was used as the fluorescently labeled dye. This MCE method takes less than 10 min of total analysis time including sample preparation and analysis of amino acids in functional foods on a PMMA chip. The results show that this approach has the potential to be a fast and simple method for amino acid analysis in functional foods.

Original languageEnglish
Pages (from-to)898-903
Number of pages6
JournalJournal of Separation Science
Volume31
Issue number5
DOIs
Publication statusPublished - Mar 1 2008
Externally publishedYes

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Electrophoresis
Amino acids
Amino Acids
Beverages
Polymethyl Methacrylate
Polymethyl methacrylates
Chromatography
Sports
Starch
Labeling
Tablets
Light emitting diodes
Coloring Agents
Dyes
Fluorescence
Functional food
Derivatives
Glass
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Cite this

Quantitative determination of amino acids in functional foods by microchip electrophoresis. / Ueno, Hiroko; Wang, Jun; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: Journal of Separation Science, Vol. 31, No. 5, 01.03.2008, p. 898-903.

Research output: Contribution to journalArticle

Ueno, Hiroko ; Wang, Jun ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Quantitative determination of amino acids in functional foods by microchip electrophoresis. In: Journal of Separation Science. 2008 ; Vol. 31, No. 5. pp. 898-903.
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