Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method

Junya Suehiro, Ryuichi Yatsunami, Ryo Hamada, Masanori Hara

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

We describe a new technique that realizes the quantitative estimation of the biological cell concentration in an aqueous medium. The proposed dielectrophoretic impedance measurement method utilizes the positive dielectrophoretic force to capture suspended biological particles onto an interdigitated micro-electrode array in pearl-chain formation. Higher cell concentration results in faster development of the pearl chains, which are electrically connected in parallel within the electrode gap to increase the conductance and capacitance between the electrodes. By monitoring temporal variation of the electrical impedance, it is possible to quantitatively evaluate the cell population according to a theoretical model of the cell collection process. It has been demonstrated that a suspension liquid of Escherichia coli could be accurately assayed in about 10 min at 105 cm-3 concentration.

Original languageEnglish
Pages (from-to)2814-2820
Number of pages7
JournalJournal of Physics D: Applied Physics
Volume32
Issue number21
DOIs
Publication statusPublished - Nov 7 1999

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

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