Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method

Junya Suehiro, Ryuichi Yatsunami, Ryo Hamada, Masanori Hara

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

We describe a new technique that realizes the quantitative estimation of the biological cell concentration in an aqueous medium. The proposed dielectrophoretic impedance measurement method utilizes the positive dielectrophoretic force to capture suspended biological particles onto an interdigitated micro-electrode array in pearl-chain formation. Higher cell concentration results in faster development of the pearl chains, which are electrically connected in parallel within the electrode gap to increase the conductance and capacitance between the electrodes. By monitoring temporal variation of the electrical impedance, it is possible to quantitatively evaluate the cell population according to a theoretical model of the cell collection process. It has been demonstrated that a suspension liquid of Escherichia coli could be accurately assayed in about 10 min at 105 cm-3 concentration.

Original languageEnglish
Pages (from-to)2814-2820
Number of pages7
JournalJournal of Physics D: Applied Physics
Volume32
Issue number21
DOIs
Publication statusPublished - Nov 7 1999

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impedance measurement
Electrodes
electrodes
Acoustic impedance
Escherichia
electrical impedance
cells
Escherichia coli
Suspensions
Capacitance
capacitance
Cells
Monitoring
Liquids
liquids

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Cite this

Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method. / Suehiro, Junya; Yatsunami, Ryuichi; Hamada, Ryo; Hara, Masanori.

In: Journal of Physics D: Applied Physics, Vol. 32, No. 21, 07.11.1999, p. 2814-2820.

Research output: Contribution to journalArticle

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