Quantitative evaluation of magnetic immunoassay with remanence measurement

Keiji Enpuku, K. Soejima, T. Nishimoto, H. Kuma, N. Hamasaki, A. Tsukamoto, K. Saitoh, A. Kandori

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10 Citations (Scopus)

Abstract

Magnetic immunoassays utilizing magnetic markers and a high -Tc SQUID have been performed. The marker was designed so as to generate remanence, and its remanence field was measured with the SQUID. The SQUID system was developed so as to measure 12 samples in one measurement sequence. We first conducted a detection of antigen called human IgE using IgE standard solution, and showed the detection of IgE down to 2 attomol. The binding process between IgE and the marker could be semi-quantitatively explained with the Langmuir-type adsorption model. We also measured IgE in human serums, and demonstrated the usefulness of the present method for practical diagnosis.

Original languageEnglish
JournalSuperconductor Science and Technology
Volume19
Issue number5
DOIs
Publication statusPublished - May 1 2006

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All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Condensed Matter Physics
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

Enpuku, K., Soejima, K., Nishimoto, T., Kuma, H., Hamasaki, N., Tsukamoto, A., ... Kandori, A. (2006). Quantitative evaluation of magnetic immunoassay with remanence measurement. Superconductor Science and Technology, 19(5). https://doi.org/10.1088/0953-2048/19/5/S19