Quantitative image analyses of nuclear dynamics in migrating neurons

Hiroki Umeshima; Yuu Kure; Kenichi Nomura; Shuhei Yoshikawa; Shinya Sakuma; Fumihito Arai; Makoto Kaneko; Mineko Kengaku

doi:10.1109/MHS.2014.7006140

Quantitative image analyses of nuclear dynamics in migrating neurons

Hiroki Umeshima, Yuu Kure, Kenichi Nomura, Shuhei Yoshikawa, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Mineko Kengaku

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.

Original language	English
Title of host publication	2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781479966790
DOIs	https://doi.org/10.1109/MHS.2014.7006140
Publication status	Published - Jan 9 2015
Externally published	Yes
Event	2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014 - Nagoya, Japan Duration: Nov 10 2014 → Nov 12 2014

Publication series

Name	2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014

Other

Other	2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014
Country/Territory	Japan
City	Nagoya
Period	11/10/14 → 11/12/14

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering
Mechanical Engineering

Access to Document

10.1109/MHS.2014.7006140

Cite this

Umeshima, H., Kure, Y., Nomura, K., Yoshikawa, S., Sakuma, S., Arai, F., Kaneko, M., & Kengaku, M. (2015). Quantitative image analyses of nuclear dynamics in migrating neurons. In 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014 [7006140] (2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MHS.2014.7006140

Quantitative image analyses of nuclear dynamics in migrating neurons. / Umeshima, Hiroki; Kure, Yuu; Nomura, Kenichi et al.

2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014. Institute of Electrical and Electronics Engineers Inc., 2015. 7006140 (2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Umeshima, H, Kure, Y, Nomura, K, Yoshikawa, S, Sakuma, S, Arai, F, Kaneko, M & Kengaku, M 2015, Quantitative image analyses of nuclear dynamics in migrating neurons. in 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014., 7006140, 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014, Institute of Electrical and Electronics Engineers Inc., 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014, Nagoya, Japan, 11/10/14. https://doi.org/10.1109/MHS.2014.7006140

Umeshima H, Kure Y, Nomura K, Yoshikawa S, Sakuma S, Arai F et al. Quantitative image analyses of nuclear dynamics in migrating neurons. In 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014. Institute of Electrical and Electronics Engineers Inc. 2015. 7006140. (2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014). doi: 10.1109/MHS.2014.7006140

@inproceedings{41d0389a06ab4c6fb7e1e77adb28ef0c,

title = "Quantitative image analyses of nuclear dynamics in migrating neurons",

abstract = "In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.",

author = "Hiroki Umeshima and Yuu Kure and Kenichi Nomura and Shuhei Yoshikawa and Shinya Sakuma and Fumihito Arai and Makoto Kaneko and Mineko Kengaku",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.; 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014 ; Conference date: 10-11-2014 Through 12-11-2014",

year = "2015",

month = jan,

day = "9",

doi = "10.1109/MHS.2014.7006140",

language = "English",

series = "2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

booktitle = "2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014",

address = "United States",

}

TY - GEN

T1 - Quantitative image analyses of nuclear dynamics in migrating neurons

AU - Umeshima, Hiroki

AU - Kure, Yuu

AU - Nomura, Kenichi

AU - Yoshikawa, Shuhei

AU - Sakuma, Shinya

AU - Arai, Fumihito

AU - Kaneko, Makoto

AU - Kengaku, Mineko

PY - 2015/1/9

Y1 - 2015/1/9

N2 - In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.

AB - In this paper, we present a novel method to investigate the mechanism driving nuclear translocation in migrating neurons via digital image analyses of the nucleus. We obtained and quantitatively analyzed time-lapse images of the fluorescently labeled nucleus with a high-speed confocal microscope. We focused on spatiotemporal dynamics of the boundary curvature of the nucleus during nuclear translocation. Our data suggest major contribution of a pulling force to nuclear translocation during neuronal migration.

UR - http://www.scopus.com/inward/record.url?scp=84961378444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961378444&partnerID=8YFLogxK

U2 - 10.1109/MHS.2014.7006140

DO - 10.1109/MHS.2014.7006140

M3 - Conference contribution

AN - SCOPUS:84961378444

T3 - 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014

BT - 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2014 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2014

Y2 - 10 November 2014 through 12 November 2014

ER -

Quantitative image analyses of nuclear dynamics in migrating neurons

Abstract

Publication series

Other

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this