Quantitative monitoring of circulating epstein-barr virus DNA for predicting the development of posttransplantation lymphoproliferative disease

Shouichi Ohga, Eiji Kubo, Akihiko Nomura, Hidetoshi Takada, Naohiro Suga, Eiichi Ishii, Aiko Suminoe, Takeshi Inamitsu, Akinobu Matsuzaki, Naoki Kasuga, Toshiro Haraa

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29 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV)-DNA was quantitatively measured to assess posttransplantation virus reactivation by realtime polymerase chain reaction (PCR). In the first retrospective analysis of a 7-year-old boy with lymphoproliferative disease (LPD) after an unrelated cord blood transplantation, serum EBV-DNA progressively increased to 4 × 105 copies/mL. EBV load was then prospectively monitored in peripheral blood from posttransplantation patients. The second case was an 8 year-old boy with aplastic anemia who received a CD34+cell transplantation. This patient died of LPD with the progression of pulmonary nodules. EBV-DNA increased to 4 × 104 copies/mL after the control of cytomegalovirus reactivation. On the other hand, EBV-DNA was undetectable (<200 copies/mL) in the series of all 58 samples from 10 patients who did not develop LPD after hematopoietic stem cell transplantation. Sequential monitoring of circulating EBV-DNA by quan-titative PCR may be a useful indicator for predicting the development of posttransplantation LPD.

Original languageEnglish
Pages (from-to)323-326
Number of pages4
JournalInternational journal of hematology
Volume73
Issue number3
DOIs
Publication statusPublished - 2001

All Science Journal Classification (ASJC) codes

  • Hematology

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    Ohga, S., Kubo, E., Nomura, A., Takada, H., Suga, N., Ishii, E., Suminoe, A., Inamitsu, T., Matsuzaki, A., Kasuga, N., & Haraa, T. (2001). Quantitative monitoring of circulating epstein-barr virus DNA for predicting the development of posttransplantation lymphoproliferative disease. International journal of hematology, 73(3), 323-326. https://doi.org/10.1007/BF02981956