Quantitative proteomic analysis of nile tilapia brain following sub-chronically exposed to toxic cyanobacteria microcystis aeruginosa

Hai Xu, Yuling Ding, Xuchun Qiu, Yohei Shimasaki, Yuji Oshima

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study, we investigated the effects of Microcytis on Nile tilapia brain. Male Nile tilapia were exposed to lyophilized Microcytis aeruginosa for 28 days. An isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach coupled with nano high-performance liquid chromatography-tandem mass spectrometry (nano HPLC-MS/MS) analysis was employed to detect and identify the differential expressed proteins. A total of 49 proteins were successfully identified and categorized into functional classes that mostly included metabolism, ubiquitin-proteasome, signal transduction and transporting, indicating that Microcytis toxicity in the fish brain is complex and diverse. Our proteomic analyses added new perspective to Microcytis neurotoxicity in aquatic organisms.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume65
Issue number2
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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