Quantum dots for labeling adipose tissue-derived stem cells

Hiroshi Yukawa, Shogo Mizufune, Chiharu Mamori, Yukimasa Kagami, Koichi Oishi, Noritada Kaji, Yukihiro Okamoto, Manabu Takeshi, Hirofumi Noguchi, Yoshinobu Baba, Michinari Hamaguchi, Nobuyuki Hamajima, Shuji Hayashi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Adipose tissue-derived stem cells (ASCs) have a self-renewing ability and can be induced to differentiate into various types of mesenchymal tissue. Because of their potential for clinical application, it has become desirable to label the cells for tracing transplanted cells and for in vivo imaging. Quantum dots (QDs) are novel inorganic probes that consist of CdSe/ZnS-core/shell semiconductor nanocrystals and have recently been explored as fluorescent probes for stem cell labeling. In this study, negatively charged QDs655 were applied for ASCs labeling, with the cationic liposome, Lipofectamine. The cytotoxicity of QDs655-Lipofectamine was assessed for ASCs. Although some cytotoxicity was observed in ASCs transfected with more than 2.0 nM of QDs655, none was observed with less than 0.8 nM. To evaluate the time dependency, the fluorescent intensity with QDs655 was observed until 24 h after transfection. The fluorescent intensity gradually increased until 2 h at the concentrations of 0.2 and 0.4 nM, while the intensity increased until 4 h at 0.8 nM. The ASCs were differentiated into both adipogenic and osteogenic cells with red fluorescence after transfection with QDs655, thus suggesting that the cells retain their potential for differentiation even after transfected with QDs655. These data suggest that QDs could be utilized for the labeling of ASCs.

Original languageEnglish
Pages (from-to)591-599
Number of pages9
JournalCell Transplantation
Volume18
Issue number5-6
Publication statusPublished - Sep 24 2009

Fingerprint

Quantum Dots
Stem cells
Labeling
Semiconductor quantum dots
Adipose Tissue
Stem Cells
Tissue
Cytotoxicity
Transfection
Liposomes
Fluorescent Dyes
Nanocrystals
Labels
Fluorescence
Semiconductor materials
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Cite this

Yukawa, H., Mizufune, S., Mamori, C., Kagami, Y., Oishi, K., Kaji, N., ... Hayashi, S. (2009). Quantum dots for labeling adipose tissue-derived stem cells. Cell Transplantation, 18(5-6), 591-599.

Quantum dots for labeling adipose tissue-derived stem cells. / Yukawa, Hiroshi; Mizufune, Shogo; Mamori, Chiharu; Kagami, Yukimasa; Oishi, Koichi; Kaji, Noritada; Okamoto, Yukihiro; Takeshi, Manabu; Noguchi, Hirofumi; Baba, Yoshinobu; Hamaguchi, Michinari; Hamajima, Nobuyuki; Hayashi, Shuji.

In: Cell Transplantation, Vol. 18, No. 5-6, 24.09.2009, p. 591-599.

Research output: Contribution to journalArticle

Yukawa, H, Mizufune, S, Mamori, C, Kagami, Y, Oishi, K, Kaji, N, Okamoto, Y, Takeshi, M, Noguchi, H, Baba, Y, Hamaguchi, M, Hamajima, N & Hayashi, S 2009, 'Quantum dots for labeling adipose tissue-derived stem cells', Cell Transplantation, vol. 18, no. 5-6, pp. 591-599.
Yukawa H, Mizufune S, Mamori C, Kagami Y, Oishi K, Kaji N et al. Quantum dots for labeling adipose tissue-derived stem cells. Cell Transplantation. 2009 Sep 24;18(5-6):591-599.
Yukawa, Hiroshi ; Mizufune, Shogo ; Mamori, Chiharu ; Kagami, Yukimasa ; Oishi, Koichi ; Kaji, Noritada ; Okamoto, Yukihiro ; Takeshi, Manabu ; Noguchi, Hirofumi ; Baba, Yoshinobu ; Hamaguchi, Michinari ; Hamajima, Nobuyuki ; Hayashi, Shuji. / Quantum dots for labeling adipose tissue-derived stem cells. In: Cell Transplantation. 2009 ; Vol. 18, No. 5-6. pp. 591-599.
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