Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells

Hiroshi Yukawa, Yukimasa Kagami, Masaki Watanabe, Koichi Oishi, Yoshitaka Miyamoto, Yukihiro Okamoto, Manabu Tokeshi, Noritada Kaji, Hirofumi Noguchi, Kenji Ono, Makoto Sawada, Yoshinobu Baba, Nobuyuki Hamajima, Shuji Hayashi

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Quantum dots (QDs) have been used to study the effects of fluorescent probes for biomolecules and cell imaging. Adipose tissue-derived stem cells, which carry a relatively lower donor site morbidity, while yielding a large number of stem cells at harvest, were transduced with QDs using the octa-arginine peptide (R8) cell-penetrating peptide (CPP). The concentration ratio of QDs:R8 of 1 × 10 4 was optimal for delivery into ASCs. No cytotoxicity was observed in ASCs transduced with less than 16 nM of QDs655. In addition, >80% of the cells could be labeled within 1 h and the fluorescent intensity was maintained at least for 2 weeks. The ASCs transduced with QDs using R8 could be differentiated into both adipogenic and osteogenic cells, thus suggesting that the cells maintained their stem cell potency. The ASCs labeled with QDs using R8 were further transplanted subcutaneously into the backs of mice or into mice through the tail vein. The labeled ASCs could be imaged with good contrast using the Maestro in vivo imaging system. These data suggested that QD labeling using R8 could be utilized for the imaging of ASCs.

Original languageEnglish
Pages (from-to)4094-4103
Number of pages10
JournalBiomaterials
Volume31
Issue number14
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

Fingerprint

Quantum Dots
Arginine
Stem cells
Labeling
Peptides
Semiconductor quantum dots
Adipose Tissue
Stem Cells
Tissue
Imaging techniques
Cell-Penetrating Peptides
Biomolecules
Cytotoxicity
Fluorescent Dyes
Imaging systems
octa-arginine peptide
Tail
Veins
Morbidity

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Yukawa, H., Kagami, Y., Watanabe, M., Oishi, K., Miyamoto, Y., Okamoto, Y., ... Hayashi, S. (2010). Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. Biomaterials, 31(14), 4094-4103. https://doi.org/10.1016/j.biomaterials.2010.01.134

Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. / Yukawa, Hiroshi; Kagami, Yukimasa; Watanabe, Masaki; Oishi, Koichi; Miyamoto, Yoshitaka; Okamoto, Yukihiro; Tokeshi, Manabu; Kaji, Noritada; Noguchi, Hirofumi; Ono, Kenji; Sawada, Makoto; Baba, Yoshinobu; Hamajima, Nobuyuki; Hayashi, Shuji.

In: Biomaterials, Vol. 31, No. 14, 01.01.2010, p. 4094-4103.

Research output: Contribution to journalArticle

Yukawa, H, Kagami, Y, Watanabe, M, Oishi, K, Miyamoto, Y, Okamoto, Y, Tokeshi, M, Kaji, N, Noguchi, H, Ono, K, Sawada, M, Baba, Y, Hamajima, N & Hayashi, S 2010, 'Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells', Biomaterials, vol. 31, no. 14, pp. 4094-4103. https://doi.org/10.1016/j.biomaterials.2010.01.134
Yukawa, Hiroshi ; Kagami, Yukimasa ; Watanabe, Masaki ; Oishi, Koichi ; Miyamoto, Yoshitaka ; Okamoto, Yukihiro ; Tokeshi, Manabu ; Kaji, Noritada ; Noguchi, Hirofumi ; Ono, Kenji ; Sawada, Makoto ; Baba, Yoshinobu ; Hamajima, Nobuyuki ; Hayashi, Shuji. / Quantum dots labeling using octa-arginine peptides for imaging of adipose tissue-derived stem cells. In: Biomaterials. 2010 ; Vol. 31, No. 14. pp. 4094-4103.
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