Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody

Katsumi Kido, Osamu Morinaga, Yukihiro Shoyama, Hiroyuki Tanaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To establish an immunoassay for baicalin (BA), a hybridoma cell line (9D6) secreting a monoclonal antibody (MAb) against BA was prepared by cell fusion with splenocytes derived from a mouse immunized with BA-bovine serum albumin (BSA) conjugate and a myeloma cell line, SP2/0-Ag14. MAb 9D6 shows specific reactivity against BA and its aglycone, baicalein, but not against other natural products. We developed an enzyme-linked immunosorbent assay (ELISA) using MAb 9D6 in a competitive manner, ranging from 200 ng/mL to 2 μg/mL. After validating the developed ELISA on the basis of intra- and inter-assays and a recovery experiment, it was found that the ELISA was not only simple, but also sufficiently reliable and accurate for quality control of Scutellariae Radix. It allowed determination of BA in complex and mixed materials, such as Kampo medicines.

Original languageEnglish
Pages (from-to)346-350
Number of pages5
JournalTalanta
Volume77
Issue number1
DOIs
Publication statusPublished - Oct 19 2008

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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