Abstract
A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.
| Original language | English |
|---|---|
| Pages (from-to) | 33-41 |
| Number of pages | 9 |
| Journal | Methods in Molecular Biology |
| Volume | 1147 |
| DOIs | |
| Publication status | Published - Jan 1 2014 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Cite this
Quorum sensing in gram-positive bacteria : Assay protocols for staphylococcal agr and enterococcal fsr systems. / Shojima, Akane; Nakayama, Jiro.
In: Methods in Molecular Biology, Vol. 1147, 01.01.2014, p. 33-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quorum sensing in gram-positive bacteria
T2 - Assay protocols for staphylococcal agr and enterococcal fsr systems
AU - Shojima, Akane
AU - Nakayama, Jiro
PY - 2014/1/1
Y1 - 2014/1/1
N2 - A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.
AB - A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.
UR - http://www.scopus.com/inward/record.url?scp=84910137272&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84910137272&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0467-9_3
DO - 10.1007/978-1-4939-0467-9_3
M3 - Article
C2 - 24664824
AN - SCOPUS:84910137272
VL - 1147
SP - 33
EP - 41
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -