Quorum sensing in gram-positive bacteria: Assay protocols for staphylococcal agr and enterococcal fsr systems

Akane Shojima, Jiro Nakayama

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.

Original languageEnglish
Pages (from-to)33-41
Number of pages9
JournalMethods in Molecular Biology
Volume1147
DOIs
Publication statusPublished - Jan 1 2014

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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