TY - JOUR
T1 - Quorum sensing in gram-positive bacteria
T2 - Assay protocols for staphylococcal agr and enterococcal fsr systems
AU - Shojima, Akane
AU - Nakayama, Jiro
PY - 2014/1/1
Y1 - 2014/1/1
N2 - A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.
AB - A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram- positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agr and fsr) of two representative gram-positive pathogens, Staphylococcus aureus and Enterococcus faecalis . These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.
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U2 - 10.1007/978-1-4939-0467-9_3
DO - 10.1007/978-1-4939-0467-9_3
M3 - Article
C2 - 24664824
AN - SCOPUS:84910137272
VL - 1147
SP - 33
EP - 41
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -