Rab27 effector Slp2-a transports the apical signaling molecule podocalyxin to the apical surface of MDCK II cells and regulates claudin-2 expression.

Takao Yasuda, Chika Saegusa, Sachiko Kamakura, Hideki Sumimoto, Mitsunori Fukuda

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Most cells in tissues are polarized and usually have two distinct plasma membrane domains-an apical membrane and a basolateral membrane, which are the result of polarized trafficking of proteins and lipids. However, the mechanism underlying the cell polarization is not fully understood. In this study, we investigated the involvement of synaptotagmin-like protein 2-a (Slp2-a), an effector molecule for the small GTPase Rab27, in polarized trafficking by using Madin-Darby canine kidney II cells as a model of polarized cells. The results show that the level of Slp2-a expression in MDCK II cells increases greatly as the cells become polarized and that its expression is specifically localized at the apical membrane. The results also reveal that Slp2-a is required for targeting of the signaling molecule podocalyxin to the apical membrane in a Rab27A-dependent manner. In addition, ezrin, a downstream target of podocalyxin, and ERK1/2 are activated in Slp2-a-knockdown cells, and their activation results in a dramatic reduction in the amount of the tight junction protein claudin-2. Because both Slp2-a and claudin-2 are highly expressed in mouse renal proximal tubules, Slp2-a is likely to regulate claudin-2 expression through trafficking of podocalyxin to the apical surface in mouse renal tubule epithelial cells.

Original languageEnglish
Pages (from-to)3229-3239
Number of pages11
JournalMolecular biology of the cell
Volume23
Issue number16
Publication statusPublished - Jan 1 2012

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Claudin-2
Synaptotagmins
Madin Darby Canine Kidney Cells
Proteins
Membranes
Zonula Occludens-2 Protein
Proximal Kidney Tubule
Monomeric GTP-Binding Proteins
Protein Transport
podocalyxin
Epithelial Cells
Cell Membrane
Kidney
Lipids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Rab27 effector Slp2-a transports the apical signaling molecule podocalyxin to the apical surface of MDCK II cells and regulates claudin-2 expression. / Yasuda, Takao; Saegusa, Chika; Kamakura, Sachiko; Sumimoto, Hideki; Fukuda, Mitsunori.

In: Molecular biology of the cell, Vol. 23, No. 16, 01.01.2012, p. 3229-3239.

Research output: Contribution to journalArticle

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