Radial alignment of microtubules through tubulin polymerization in an evaporating droplet

Jakia Jannat Keya, Hiroki Kudoh, Arif Md Rashedul Kabir, Daisuke Inoue, Nobuyoshi Miyamoto, Tomomi Tani, Akira Kakugo, Kazuhiro Shikinaka

Research output: Contribution to journalArticle

Abstract

We report the formation of spherulites from droplets of highly concentrated tubulin solution via nucleation and subsequent polymerization to microtubules (MTs) under water evaporation by heating. Radial alignment of MTs in the spherulites was confirmed by the optical properties of the spherulites observed using polarized optical microscopy and fluorescence microscopy. Temperature and concentration of tubulins were found as important parameters to control the spherulite pattern formation of MTs where evaporation plays a significant role. The alignment of MTs was regulated reversibly by temperature induced polymerization and depolymerization of tubulins. The formation of the MTs patterns was also confirmed at the molecular level from the small angle X-ray measurements. This work provides a simple method for obtaining radially aligned arrays of MTs.

Original languageEnglish
Article numbere0231352
JournalPloS one
Volume15
Issue number4
DOIs
Publication statusPublished - Apr 2020

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Radial alignment of microtubules through tubulin polymerization in an evaporating droplet'. Together they form a unique fingerprint.

  • Cite this

    Keya, J. J., Kudoh, H., Rashedul Kabir, A. M., Inoue, D., Miyamoto, N., Tani, T., Kakugo, A., & Shikinaka, K. (2020). Radial alignment of microtubules through tubulin polymerization in an evaporating droplet. PloS one, 15(4), [e0231352]. https://doi.org/10.1371/journal.pone.0231352