Radiation-inducible TNF-α gene expression under stress-inducible promoter gadd 153 for cancer therapy

Akira Ito, Masashige Shinkai, Kazumi Hakamada, Hiroyuki Honda, Takeshi Kobayashi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We demonstrated the effectiveness of radiation-inducible expression of the TNF-α gene for cancer therapy in vitro. The TNF-α gene under the control of the stress-inducible promoter, gadd 153, was introduced into the human glioma cell line, U251-SP. Without cobalt-60 gamma irradiation, no cytotoxicity against the transfected cells was observed. When the transfected cells were irradiated with 10 or 20 gray (Gy), the gadd 153 promoter was highly induced and the expression level of TNF-α increased. Five days after the irradiation, the TNF-α productions of each cell irradiated with 10 and 20 Gy were 30 and 100 times higher than the basal level, respectively. The cytotoxicities against the transfected cells 5 d after irradiation with 10 and 20 Gy were 79% or 91%, respectively, which are much higher than those against the nontransfected cells that were irradiated at the same dose (43% and 78%, respectively). These results demonstrate that the gadd 153-TNF-α system may be an effective tool for radiosurgery of malignant brain tumors.

Original languageEnglish
Pages (from-to)598-601
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume92
Issue number6
DOIs
Publication statusPublished - Jan 1 2001

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Gene expression
Irradiation
Radiation
Cytotoxicity
Gene Expression
Genes
Cells
Neoplasms
Cobalt
Tumors
Brain
Therapeutics
Radiosurgery
Brain Neoplasms
Glioma
Genetic Therapy
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Radiation-inducible TNF-α gene expression under stress-inducible promoter gadd 153 for cancer therapy. / Ito, Akira; Shinkai, Masashige; Hakamada, Kazumi; Honda, Hiroyuki; Kobayashi, Takeshi.

In: Journal of Bioscience and Bioengineering, Vol. 92, No. 6, 01.01.2001, p. 598-601.

Research output: Contribution to journalArticle

Ito, Akira ; Shinkai, Masashige ; Hakamada, Kazumi ; Honda, Hiroyuki ; Kobayashi, Takeshi. / Radiation-inducible TNF-α gene expression under stress-inducible promoter gadd 153 for cancer therapy. In: Journal of Bioscience and Bioengineering. 2001 ; Vol. 92, No. 6. pp. 598-601.
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