TY - JOUR
T1 - Radioiodinated Nitroxide Derivative for the Detection of Lipid Radicals
AU - Yamasaki, Toshihide
AU - Azuma, Risa
AU - Sano, Kohei
AU - Munekane, Masayuki
AU - Matsuoka, Yuta
AU - Yamada, Ken Ichi
AU - Mukai, Takahiro
N1 - Funding Information:
T.Y. and R.A. contributed equally. This study was supported in part by AMED CREST grant number JP19gm0910013 (K.Y.), by JSPS KAKENHI grant numbers 19K17283 (T.Y.), 19H03607 (T.M.), 18K19405 (K.Y.), and 17H03977 (K.Y.), and by Hyogo Science and Technology Association (T.Y.). The authors declare no competing financial interest.
PY - 2020/1/9
Y1 - 2020/1/9
N2 - Thus far, no accurate measurement technology has been developed to detect lipid alkyl radicals (lipid radicals), which cause lipid peroxidation. Therefore, we aimed to develop a nuclear medical imaging probe that can be taken up in the lipophilic site in cells such as biological membranes, by reacting specifically with the lipid radicals generated there. We designed and synthesized 4-(4-[125I]iodobenzamido)-2,2,6,6-tetramethylpiperidine-1-oxyl, which shows high reactivity to lipid radicals with a high radiochemical yield and purity. Intracellular retention was found to increase significantly when lipid radicals were produced.
AB - Thus far, no accurate measurement technology has been developed to detect lipid alkyl radicals (lipid radicals), which cause lipid peroxidation. Therefore, we aimed to develop a nuclear medical imaging probe that can be taken up in the lipophilic site in cells such as biological membranes, by reacting specifically with the lipid radicals generated there. We designed and synthesized 4-(4-[125I]iodobenzamido)-2,2,6,6-tetramethylpiperidine-1-oxyl, which shows high reactivity to lipid radicals with a high radiochemical yield and purity. Intracellular retention was found to increase significantly when lipid radicals were produced.
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U2 - 10.1021/acsmedchemlett.9b00416
DO - 10.1021/acsmedchemlett.9b00416
M3 - Article
AN - SCOPUS:85076596486
VL - 11
SP - 45
EP - 48
JO - ACS Medicinal Chemistry Letters
JF - ACS Medicinal Chemistry Letters
SN - 1948-5875
IS - 1
ER -