Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells

Hideya Ohnishi, Akifumi Kiyota, Norihiro Koya, Hiroto Tanaka, Masayo Umebayashi, Mitsuo Katano, Takashi Morisaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Activated lymphocytes have the ability to undergo non-directional cell movement known as random migration, although the biological role for this remains unclear. Herein, we investigated how random migration affects cytotoxicity of activated lymphocytes using time-lapse imaging analysis. The kinetics of random migration paralleled cytotoxicity in activated lymphocytes. Sphingosine-1-phosphate (S1P) and its receptor-1 (S1PR1) play an important role in lymphocyte migration. Phosphorylated FTY720 (FTYP), a structural analog of S1P, significantly inhibited random migration and cytotoxicity of activated CD3+NKG2D+ CD8+ T-lymphocytes but not CD3-NKG2D+CD56+ natural killer (NK) cells. In a mouse xenograft model, FTYP-treated activated lymphocytes exhibited lower cytotoxicity and less tumor infiltration for activated CD3+ NKG2D+ T-lymphocytes but not CD3-NKG2D+ NK cells. These results suggest that random migration contributes to the cytotoxicity of activated CD8+ T-cells but not of NK cells.

Original languageEnglish
Pages (from-to)3947-3956
Number of pages10
JournalAnticancer research
Volume34
Issue number8
Publication statusPublished - Aug 1 2014

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Natural Killer Cells
Lymphocytes
T-Lymphocytes
Time-Lapse Imaging
Lysosphingolipid Receptors
Heterografts
Cell Movement
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Ohnishi, H., Kiyota, A., Koya, N., Tanaka, H., Umebayashi, M., Katano, M., & Morisaki, T. (2014). Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells. Anticancer research, 34(8), 3947-3956.

Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells. / Ohnishi, Hideya; Kiyota, Akifumi; Koya, Norihiro; Tanaka, Hiroto; Umebayashi, Masayo; Katano, Mitsuo; Morisaki, Takashi.

In: Anticancer research, Vol. 34, No. 8, 01.08.2014, p. 3947-3956.

Research output: Contribution to journalArticle

Ohnishi, H, Kiyota, A, Koya, N, Tanaka, H, Umebayashi, M, Katano, M & Morisaki, T 2014, 'Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells', Anticancer research, vol. 34, no. 8, pp. 3947-3956.
Ohnishi H, Kiyota A, Koya N, Tanaka H, Umebayashi M, Katano M et al. Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells. Anticancer research. 2014 Aug 1;34(8):3947-3956.
Ohnishi, Hideya ; Kiyota, Akifumi ; Koya, Norihiro ; Tanaka, Hiroto ; Umebayashi, Masayo ; Katano, Mitsuo ; Morisaki, Takashi. / Random migration contributes to cytotoxicity of activated CD8+ T-lymphocytes but not NK cells. In: Anticancer research. 2014 ; Vol. 34, No. 8. pp. 3947-3956.
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