RAPD Analysis of Genotoxic Effects of Nano-Scale SiO2and TiO2on Broad Bean (Vicia Faba L.)

Ola A. Galal, Ahmad F. Thabet, Midori Tuda, Magdy F.M. El-SAMAHY

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Growing use of oxide nanoparticles has driven their access to the natural environment, including biological interactions within the ecosystem, despite a lack of knowledge about their genotoxic potential on exposed plant tissue. In this study, seeds of broad beans (Vicia faba L.), one of the major carbohydrate food sources as well as an ecotoxicological model plant, were treated with three concentrations (25, 50 and 75 mg/L) of two different types of nanoscale materials (n-), n-SiO2and n-TiO2, to assess their safe use. Seeds were soaked in n-SiO2and n-TiO2each for 24 h, then germinated in peat moss for two weeks. DNA was isolated from leaves for RAPD (Random Amplified Polymorphic DNA) profile analyses.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume65
Issue number1
DOIs
Publication statusPublished - 2020

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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