Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography

Takeshi Bamba, Ei Ichiro Fukusaki, Yoshihisa Nakazawa, Akio Kobayashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.

Original languageEnglish
Pages (from-to)293-296
Number of pages4
JournalJournal of Separation Science
Volume27
Issue number4
DOIs
Publication statusPublished - Mar 1 2004
Externally publishedYes

Fingerprint

High performance liquid chromatography
Silicon Dioxide
Silica

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Cite this

Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography. / Bamba, Takeshi; Fukusaki, Ei Ichiro; Nakazawa, Yoshihisa; Kobayashi, Akio.

In: Journal of Separation Science, Vol. 27, No. 4, 01.03.2004, p. 293-296.

Research output: Contribution to journalArticle

@article{486ae5e27606416ba0ffe6f2b722ed02,
title = "Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography",
abstract = "A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.",
author = "Takeshi Bamba and Fukusaki, {Ei Ichiro} and Yoshihisa Nakazawa and Akio Kobayashi",
year = "2004",
month = "3",
day = "1",
doi = "10.1002/jssc.200301664",
language = "English",
volume = "27",
pages = "293--296",
journal = "Journal of Separation Science",
issn = "1615-9306",
publisher = "Wiley-VCH Verlag",
number = "4",

}

TY - JOUR

T1 - Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography

AU - Bamba, Takeshi

AU - Fukusaki, Ei Ichiro

AU - Nakazawa, Yoshihisa

AU - Kobayashi, Akio

PY - 2004/3/1

Y1 - 2004/3/1

N2 - A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.

AB - A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.

UR - http://www.scopus.com/inward/record.url?scp=1842435969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842435969&partnerID=8YFLogxK

U2 - 10.1002/jssc.200301664

DO - 10.1002/jssc.200301664

M3 - Article

C2 - 15334916

AN - SCOPUS:1842435969

VL - 27

SP - 293

EP - 296

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 4

ER -