Rapid and high-resolution analysis of geometric polyprenol homologues by connected octadecylsilylated monolithic silica columns in high-performance liquid chromatography

Takeshi Bamba, Ei Ichiro Fukusaki, Yoshihisa Nakazawa, Akio Kobayashi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A Chromolith Performance octadecylsilyl (ODS) monolithic silica column (Merck) was compared with a conventional microparticulate ODS-bonded silica column in the high-performance liquid chromatography separation of natural polyprenols. A system comprising two connected monolithic columns afforded an equivalent separation at half the analysis time of the conventional method. Furthermore, ten connected columns achieved a tremendously high-resolution separation, in which the complicated series of homologous polyprenols with geometric isomerism were fully separated.

Original languageEnglish
Pages (from-to)293-296
Number of pages4
JournalJournal of Separation Science
Volume27
Issue number4
DOIs
Publication statusPublished - Mar 1 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

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