Rapid and serum-insensitive endocytotic delivery of proteins using biotinylated polymers attached via multivalent hydrophobic anchors

Kyohei Tobinaga, Cuicui Li, Masafumi Takeo, Masayoshi Matsuda, Hiroko Nagai, Takuro Niidome, Tatsuhiro Yamamoto, Akihiro Kishimura, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have designed biotinylated polymers as synthetic receptors that have multiple alkyl groups for endocytotic delivery of target proteins. The polymers were stably attached to a cell surface via multivalent anchoring. The presented biotin was bound to streptavidin (SA) on the cell surface, and, via an endocytotic pathway, the cell rapidly internalized the biotinylated polymer/SA complex. The cell's uptake of the complex was not inhibited by the presence of 10% fetal bovine serum, and its efficacy for the uptake of SA was the highest when compared with commercial reagents and single-anchored-type synthetic receptors. The synthetic receptor-mediated endocytosis can be used generally for other kind of protein by using SA as an adaptor molecule between a target protein and the cell-surface presented biotin.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalJournal of Controlled Release
Volume177
Issue number1
DOIs
Publication statusPublished - Mar 10 2014

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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