Rapid detection of FKS-associated echinocandin resistance in Candida glabrata

Yanan Zhao, Yoji Nagasaki, Milena Kordalewska, Ellen G. Press, Ryan K. Shields, M. Hong Nguyen, Cornelius J. Clancy, David S. Perlin

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

A novel and highly accurate diagnostic assay platform was established for rapid identification of FKS mutations associated with echinocandin resistance in Candida glabrata. The assay platform uses allele-specific molecular beacon and DNA melt analysis following asymmetric PCR. A dual assay for FKS1 and FKS2 was developed to identify within 3 h the most common and clinically relevant resistance-associated mutations, including 8 FKS1 HS1 (wild type [WT], S629P, F625S, D632Y, D632E [T1896G], D632E [T1896A], I634V, and F625F) and 7 FKS2 HS1 (WT, F659del, F659S, F659V, F659L, S663P, and S663F) genotypes. A blinded panel of 188 C. glabrata clinical isolates was tested by both assays. The molecular diagnostic results from the dual assay were 100% concordant with data obtained from DNA sequencing. This platform has the potential to overcome the deficiencies of existing in vitro susceptibility-based assays to identify echinocandin-resistant C. glabrata and holds promise as a surrogate diagnostic method to better direct echinocandin therapy.

Original languageEnglish
Pages (from-to)6573-6577
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume60
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Rapid detection of FKS-associated echinocandin resistance in Candida glabrata'. Together they form a unique fingerprint.

Cite this