Rapid enzyme-linked immunosorbent assay with functional magnetite particles

Masashige Shinkai; Jin Wang; Masamichi Kamihira; Masashi Iwata; Hiroyuki Honda; Takeshi Kobayashi

doi:10.1016/0922-338X(92)90683-L

Rapid enzyme-linked immunosorbent assay with functional magnetite particles

Masashige Shinkai, Jin Wang, Masamichi Kamihira, Masashi Iwata, Hiroyuki Honda, Takeshi Kobayashi

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.

Original language	English
Pages (from-to)	166-168
Number of pages	3
Journal	Journal of Fermentation and Bioengineering
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/0922-338X(92)90683-L
Publication status	Published - 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology

Access to Document

10.1016/0922-338X(92)90683-L

Cite this

@article{6dacc8d0a36449da9858e2c1a9624651,

title = "Rapid enzyme-linked immunosorbent assay with functional magnetite particles",

abstract = "Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.",

author = "Masashige Shinkai and Jin Wang and Masamichi Kamihira and Masashi Iwata and Hiroyuki Honda and Takeshi Kobayashi",

year = "1992",

doi = "10.1016/0922-338X(92)90683-L",

language = "English",

volume = "73",

pages = "166--168",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

number = "2",

}

TY - JOUR

T1 - Rapid enzyme-linked immunosorbent assay with functional magnetite particles

AU - Shinkai, Masashige

AU - Wang, Jin

AU - Kamihira, Masamichi

AU - Iwata, Masashi

AU - Honda, Hiroyuki

AU - Kobayashi, Takeshi

PY - 1992

Y1 - 1992

N2 - Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.

AB - Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5 μg/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.

UR - http://www.scopus.com/inward/record.url?scp=0026545046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026545046&partnerID=8YFLogxK

U2 - 10.1016/0922-338X(92)90683-L

DO - 10.1016/0922-338X(92)90683-L

M3 - Article

AN - SCOPUS:0026545046

SN - 1389-1723

VL - 73

SP - 166

EP - 168

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 2

ER -

Rapid enzyme-linked immunosorbent assay with functional magnetite particles

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this