Rapid Extraction-HPLC as a Tool for Presumptive Identification of Burkholderia gladioli, B. glumae and B. plantarii, Causal Agents of Various Rice Diseases

Nobuaki Matsuyama, Yasuko Ueda, Kazuhiro Iiyama, Naruto Furuya, Hiroyuki Ura, Abu Ashraf Khan, Matsumoto Masaru

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Rapid extraction-HPLC was performed to estimate its usefulness for presumptive identification of Burkholderia gladioli, B. glumae and B. plantarii. On HPLC chromatograms of 12 isolates of B. gladioli, a distinct peak appeared at Rt 6.2 min without exception. This peak was absent on the chromatograms for 18 isolates of B. glumae and 5 isolates of B. plantarii tested. Benchmark peak at Rt 3.2min was observed on the chromatogram of B. plantarii. Together with the direct colony TLC method, this simplified HPLC analysis will be useful for identification of phytopathogenic bacteria.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume42
Issue number3-4
Publication statusPublished - Mar 1 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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