Rapid fluorescent detection of (anti)androgens with spiggin-gfp medaka

Anthony Sébillot, Pauliina Damdimopoulou, Yukiko Ogino, Petra Spirhanzlova, Shinichi Miyagawa, David Du Pasquier, Nora Mouatassim, Taisen Iguchi, Gregory F. Lemkine, Barbara A. Demeneix, Andrew J. Tindall

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Widespread environmental antiandrogen contamination has been associated with negative impacts on biodiversity and human health. In particular, many pesticides are antiandrogenic, creating a need for robust and sensitive environmental monitoring. Our aim was to develop a sensitive and specific transgenic medaka (Oryzias latipes) model bearing an androgen responsive fluorescent reporter construct for whole organism-based environmental screening of pro- and antiandrogens. We analyzed the 5' regions of the androgen responsive threespined stickleback (Gasterosteus aculeatus) spiggin genes in silico, revealing conserved blocks of sequence harboring androgen response elements. Identified putative promoters were cloned upstream of GFP. Germinal transgenesis with spg1-gfp led to stable medaka lines. GFP induction was exclusive to the kidney, the site of spiggin protein production in sticklebacks. Significant GFP expression was induced by three or four-day androgen treatment of newly hatched fry, but not by estrogens, mineralocorticoids, glucocorticoids or progestogens. The model responded dose-dependently to androgens, with highest sensitivity to 17MT (1.5 μg/L). In addition to flutamide, the biocides fenitrothion, vinclozolin and linuron significantly inhibited 17MT-induced GFP induction, validating the model for detection of antiandrogens. The spg1-gfp medaka model provides a sensitive, specific, and physiologically pertinent biosensor system for analyzing environmental androgen activity.

Original languageEnglish
Pages (from-to)10919-10928
Number of pages10
JournalEnvironmental Science and Technology
Volume48
Issue number18
DOIs
Publication statusPublished - Sep 16 2014
Externally publishedYes

Fingerprint

androgen
Androgens
Androgen Antagonists
vinclozolin
Linuron
Bearings (structural)
pesticide
Fenitrothion
Flutamide
fenitrothion
Mineralocorticoids
Disinfectants
Biodiversity
Response Elements
Progestins
environmental monitoring
Pesticides
Biosensors
Glucocorticoids
spiggin

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Sébillot, A., Damdimopoulou, P., Ogino, Y., Spirhanzlova, P., Miyagawa, S., Du Pasquier, D., ... Tindall, A. J. (2014). Rapid fluorescent detection of (anti)androgens with spiggin-gfp medaka. Environmental Science and Technology, 48(18), 10919-10928. https://doi.org/10.1021/es5030977

Rapid fluorescent detection of (anti)androgens with spiggin-gfp medaka. / Sébillot, Anthony; Damdimopoulou, Pauliina; Ogino, Yukiko; Spirhanzlova, Petra; Miyagawa, Shinichi; Du Pasquier, David; Mouatassim, Nora; Iguchi, Taisen; Lemkine, Gregory F.; Demeneix, Barbara A.; Tindall, Andrew J.

In: Environmental Science and Technology, Vol. 48, No. 18, 16.09.2014, p. 10919-10928.

Research output: Contribution to journalArticle

Sébillot, A, Damdimopoulou, P, Ogino, Y, Spirhanzlova, P, Miyagawa, S, Du Pasquier, D, Mouatassim, N, Iguchi, T, Lemkine, GF, Demeneix, BA & Tindall, AJ 2014, 'Rapid fluorescent detection of (anti)androgens with spiggin-gfp medaka', Environmental Science and Technology, vol. 48, no. 18, pp. 10919-10928. https://doi.org/10.1021/es5030977
Sébillot A, Damdimopoulou P, Ogino Y, Spirhanzlova P, Miyagawa S, Du Pasquier D et al. Rapid fluorescent detection of (anti)androgens with spiggin-gfp medaka. Environmental Science and Technology. 2014 Sep 16;48(18):10919-10928. https://doi.org/10.1021/es5030977
Sébillot, Anthony ; Damdimopoulou, Pauliina ; Ogino, Yukiko ; Spirhanzlova, Petra ; Miyagawa, Shinichi ; Du Pasquier, David ; Mouatassim, Nora ; Iguchi, Taisen ; Lemkine, Gregory F. ; Demeneix, Barbara A. ; Tindall, Andrew J. / Rapid fluorescent detection of (anti)androgens with spiggin-gfp medaka. In: Environmental Science and Technology. 2014 ; Vol. 48, No. 18. pp. 10919-10928.
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