Rapid, highly sensitive, and simultaneous detection of staphylococcal enterotoxins in milk by using immuno-pillar devices

Toshihiro Kasama, Mai Ikami, Wanchun Jin, Keiko Yamada, Noritada Kaji, Yusuke Atsumi, Makoto Mizutani, Atsushi Murai, Akira Okamoto, Takao Namikawa, Michio Ohta, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Staphylococcal enterotoxins (SEs) have repeatedly caused food poisoning incidents worldwide. Some of the challenges associated with food poisoning outbreaks are that traditional detection methods are expensive and require long processing times and trained technicians. Microchannel devices represent a potential detection method by which these difficulties can be overcome. In this paper, we propose that immuno-pillar devices may represent a rapid, highly sensitive, and low-cost analytical system for the simultaneous detection of staphylococcal enterotoxin types A, B, and D (SEA, SEB, and SED) in milk. To prepare milk samples simulating food contaminated with SEs, commercial milk was spiked with equal amounts of SEA, SEB, and SED. A quantitative analysis of the milk samples was performed within 15 min by using the microchannel device. The analysis required only 0.5 μL of untreated milk sample. The resultant limit of detection was 15.6 pg mL-1 for each SE, and the total assay time and sensitivity were markedly shorter and higher, respectively, than those for commercially available assay kits. The detection range of each enterotoxin using these devices was estimated as 15.6 pg mL-1 to 100 ng mL-1, which completely covers the SE concentrations that can lead to foodborne diseases based on the U.S. Food and Drug Administration's criterion for the infectious SE dose in SE poisoning (1 μg SE). Using our devices, frequent assessment of food potentially contaminated with SE is possible.

Original languageEnglish
Pages (from-to)5092-5095
Number of pages4
JournalAnalytical Methods
Issue number12
Publication statusPublished - Jun 21 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)


Dive into the research topics of 'Rapid, highly sensitive, and simultaneous detection of staphylococcal enterotoxins in milk by using immuno-pillar devices'. Together they form a unique fingerprint.

Cite this