Rapid hyperthermic cell sensitivity test measured by RNA synthesis using contact-sensitive plates of confluent balb/c 3t3 cell monolayers

H. Matsuoka, K. Sugimachi, H. Ueo, M. Mori, T. Akiyoshi

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed a hyperthermic sensitivity test for human neoplastic cells using contact-sensitive confluent monolayers of BALB/c 3T3 cells designated as 'contact-sensitive plates (CSP)' which almost completely inhibited the growth of normal cells without influencing the growth of the human neoplastic cells. On 'CSP' the relative [3H]uridine incorporation into RNA of the hyperthermic cultures showed an excellent correlation with the relative clonogenic efficiency of the treated cultures. Since 'CSP' allows for only contact-insensitive tumour cells to proliferate, our assay will predict the hyperthermic sensitivity of only the neoplastic cells. Moreover, even 2 × 103 cells derived from clinical tumour tissues can be assayed within only 3 days. This assay system is potentially used as a rapid in vitro hyperthermic sensitivity test for human carcinoma.

Original languageEnglish
Pages (from-to)1019-1029
Number of pages11
JournalInternational Journal of Hyperthermia
Volume6
Issue number6
DOIs
Publication statusPublished - 1990

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)
  • Cancer Research

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