Rapid increase in clusters of synaptophysin at onset of homosynaptic potentiation in Aplysia

Iksung Jin, Hiroshi Udo, Robert D. Hawkins

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Imaging studies have shown that even the earliest phases of longterm plasticity are accompanied by the rapid recruitment of synaptic components, which generally requires actin polymerization and may be one of the first steps in a program that can lead to the formation of new stable synapses during late-phase plasticity. However, most of those results come from studies of long-term potentiation in rodent hippocampus and might not generalize to other forms of synaptic plasticity or plasticity in other brain areas and species. For example, recruitment of presynaptic proteins during long-term facilitation by 5HT in Aplysia is delayed for several hours, suggesting that whereas activity-dependent forms of plasticity, such as long-term potentiation, involve rapid recruitment of presynaptic proteins, neuromodulatory forms of plasticity, such as facilitation by 5HT, involve more delayed recruitment. To begin to explore this hypothesis, we examined an activity-dependent form of plasticity, homosynaptic potentiation produced by tetanic stimulation of the presynaptic neuron in Aplysia.We found that homosynaptic potentiation involves presynaptic but not postsynaptic actin and a rapid (under 10 min) increase in the number of clusters of the presynaptic vesicle-associated protein synaptophysin. These results indicate that rapid recruitment of synaptic components is not limited to hippocampal potentiation and support the hypothesis that activity-dependent types of plasticity involve rapid recruitment of presynaptic proteins, whereas neuromodulatory types of plasticity involve more delayed recruitment.

Original languageEnglish
Pages (from-to)11656-11661
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number28
DOIs
Publication statusPublished - Jul 12 2011

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Aplysia
Synaptophysin
Long-Term Potentiation
Actins
Proteins
Neuronal Plasticity
Polymerization
Synapses
Rodentia
Hippocampus
Neurons
Brain

All Science Journal Classification (ASJC) codes

  • General

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Rapid increase in clusters of synaptophysin at onset of homosynaptic potentiation in Aplysia. / Jin, Iksung; Udo, Hiroshi; Hawkins, Robert D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 28, 12.07.2011, p. 11656-11661.

Research output: Contribution to journalArticle

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