TY - JOUR
T1 - Rapid microbead-based DNA detection using dielectrophoresis and impedance measurement
AU - Nakano, Michihiko
AU - Ding, Zhenhao
AU - Kasahara, Hiromichi
AU - Suehiro, Junya
N1 - Publisher Copyright:
Copyright © EPLA, 2014.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. The authors propose a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labeling. DNA-labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine ones are not. DEP-trapped microbeads are measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. The validity of the proposed method was demonstrated by detection of PCR-amplified DNA of viruses.
AB - Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. The authors propose a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labeling. DNA-labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine ones are not. DEP-trapped microbeads are measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. The validity of the proposed method was demonstrated by detection of PCR-amplified DNA of viruses.
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U2 - 10.1209/0295-5075/108/28003
DO - 10.1209/0295-5075/108/28003
M3 - Article
AN - SCOPUS:84908431661
VL - 108
JO - Journal de Physique (Paris), Lettres
JF - Journal de Physique (Paris), Lettres
SN - 0295-5075
IS - 2
M1 - 28003
ER -