Abstract
Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. The authors propose a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labeling. DNA-labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine ones are not. DEP-trapped microbeads are measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. The validity of the proposed method was demonstrated by detection of PCR-amplified DNA of viruses.
| Original language | English |
|---|---|
| Article number | 28003 |
| Journal | EPL |
| Volume | 108 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 1 2014 |
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All Science Journal Classification (ASJC) codes
- Physics and Astronomy(all)
Cite this
Rapid microbead-based DNA detection using dielectrophoresis and impedance measurement. / Nakano, Michihiko; Ding, Zhenhao; Kasahara, Hiromichi; Suehiro, Junya.
In: EPL, Vol. 108, No. 2, 28003, 01.10.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid microbead-based DNA detection using dielectrophoresis and impedance measurement
AU - Nakano, Michihiko
AU - Ding, Zhenhao
AU - Kasahara, Hiromichi
AU - Suehiro, Junya
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. The authors propose a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labeling. DNA-labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine ones are not. DEP-trapped microbeads are measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. The validity of the proposed method was demonstrated by detection of PCR-amplified DNA of viruses.
AB - Polymerase chain reaction (PCR) is a powerful tool for diagnostic procedures in bacterial and viral infections. The authors propose a new electrical technique for rapid detection of DNA amplified by PCR using dielectrophoresis (DEP) of microbeads. The method is based on dramatic alteration of DEP characteristics of microbeads caused by DNA labeling. DNA-labeled microbeads are trapped on a microelectrode under the action of positive DEP, whereas pristine ones are not. DEP-trapped microbeads are measured impedimetrically to realize rapid and quantitative detection of the amplified DNA. The validity of the proposed method was demonstrated by detection of PCR-amplified DNA of viruses.
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UR - http://www.scopus.com/inward/citedby.url?scp=84908431661&partnerID=8YFLogxK
U2 - 10.1209/0295-5075/108/28003
DO - 10.1209/0295-5075/108/28003
M3 - Article
VL - 108
JO - Europhysics Letters
JF - Europhysics Letters
SN - 0295-5075
IS - 2
M1 - 28003
ER -