Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles

Masashige Shinkai; Masamichi Kamihira; Hiroyuki Honda; Takeshi Kobayashi

doi:10.1252/kakoronbunshu.18.256

Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles

Masashige Shinkai, Masamichi Kamihira, Hiroyuki Honda, Takeshi Kobayashi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.

Original language	English
Pages (from-to)	256-259
Number of pages	4
Journal	kagaku kogaku ronbunshu
Volume	18
Issue number	2
DOIs	https://doi.org/10.1252/kakoronbunshu.18.256
Publication status	Published - Jan 1 1992
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.1252/kakoronbunshu.18.256

Fingerprint Dive into the research topics of 'Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles'. Together they form a unique fingerprint.

Cite this

@article{7bc4563f267f450caa73c560843a7ece,

title = "Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles",

abstract = "We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.",

author = "Masashige Shinkai and Masamichi Kamihira and Hiroyuki Honda and Takeshi Kobayashi",

year = "1992",

month = jan,

day = "1",

doi = "10.1252/kakoronbunshu.18.256",

language = "English",

volume = "18",

pages = "256--259",

journal = "Kagaku Kogaku Ronbunshu",

issn = "0386-216X",

publisher = "The Society of Chemical Engineers, Japan",

number = "2",

}

TY - JOUR

T1 - Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles

AU - Shinkai, Masashige

AU - Kamihira, Masamichi

AU - Honda, Hiroyuki

AU - Kobayashi, Takeshi

PY - 1992/1/1

Y1 - 1992/1/1

N2 - We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.

AB - We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.

UR - http://www.scopus.com/inward/record.url?scp=0005803671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0005803671&partnerID=8YFLogxK

U2 - 10.1252/kakoronbunshu.18.256

DO - 10.1252/kakoronbunshu.18.256

M3 - Article

AN - SCOPUS:0005803671

VL - 18

SP - 256

EP - 259

JO - Kagaku Kogaku Ronbunshu

JF - Kagaku Kogaku Ronbunshu

SN - 0386-216X

IS - 2

ER -