Rapid Purification of Monoclonal Antibody with Functional Magnetite Particles

Masashige Shinkai, Masamichi Kamihira, Hiroyuki Honda, Takeshi Kobayashi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We have developed a rapid method of purifying monoclonal antibody with functional magnetite particles. Protein A, which has an affinity to IgG-type antibody, was immobilized on magnetite particles. The affinity constant of protein A-magnetite to mouse IgG was 9×l06 l/mol. Using such particles, purified antibody of 73% purity was obtained with 60% recovery from mouse hybridoma culture broth. These affinity particles could be regenerated and reused. Using magnetite particles for affinity purification, total operation time was shortened to about 20 min.

Original languageEnglish
Pages (from-to)256-259
Number of pages4
Journalkagaku kogaku ronbunshu
Volume18
Issue number2
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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