Rapid redistribution of the postsynaptic density protein PSD-Zip45 (Homer 1c) and its differential regulation by NMDA receptors and calcium channels

Shigeo Okabe, Tomoe Urushido, Daijiro Konno, Haruo Okado, Kenji Sobue

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)

Abstract

PSD-Zip45 (Homer 1c) and PSD-95 are postsynaptic density (PSD) proteins containing distinct protein-interacting motifs. Green fluorescent protein (GFP)-tagged PSD-Zip45 and PSD-95 molecules were targeted to the PSD in hippocampal neurons. We analyzed dynamic behavior of these GFP-tagged PSD proteins by using time-lapse confocal microscopy. In contrast to the less dynamic properties of PSD-95, PSD-Zip45 showed rapid redistribution and a higher steady-state turnover rate. Differential stimulation protocols were found to alter the direction of PSD-Zip45 assembly-disassembly. Transient increases in intracellular Ca2+ by voltage-dependent Ca2+ channel activation induced PSD-Zip45 clustering. In contrast, NMDA receptor-dependent Ca2+ influx resulted in the disassembly of PSD-Zip45 clusters. Thus, neuronal activity differentially redistributes a specific subset of PSD proteins, which are important for localization of both surface receptors and intracellular signaling complexes.

Original languageEnglish
Pages (from-to)9561-9571
Number of pages11
JournalJournal of Neuroscience
Volume21
Issue number24
DOIs
Publication statusPublished - Dec 15 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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