Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR

Kazuoki Osumi, Takafumi Fukui, Hitoshi Kiyoi, Masanobu Kasai, Yoshihisa Kodera, Kazuko Kudo, Koji Kato, Takaharu Matsuyama, Kazuyuki Naito, Mitsune Tanimoto, Hisamaru Hirai, Hidehiko Saito, Ryuzo Ohno, Tomoki Naoe

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We established a real-time PCR method that can simultaneously detect 10 different fusion transcripts (major, minor and micro BCR/ABL, AML1/MTG8, PML/RARα, CBFβ/MYH11, TEL/AML1, E2A/PBX1, MLL/AF4, and MLL/AF9) together with Wilms' tumor gene (WT1) transcripts. This screening method allowed the processing of six specimens concomitantly and required only one working day from RNA extraction to final results. Fifty-seven bone marrow (BM) samples from patients with acute leukemia were retrospectively screened for the presence of fusion and WT1 transcripts without knowledge of the cytogenetic data, and the fusion transcripts were detected in 20 of 57 samples (35.1%). The concordance between the present method and cytogenetic analysis was examined in 38 samples in which the cytogenetic data were available. In 12 of 38 samples, the PCR results agreed with the cytogenetic data, whereas in 4 of the remaining 26 samples, the translocations were detected by real-time PCR alone because of the insufficient number of metaphases obtained and presumably the submicroscopic or masked translocations. The WT1 levels ranged from 400 to 690,000 copies/μg RNA in BM from leukemia patients, whereas 0-470 copies/μg RNA were found in BM cells from BMT donors. This real-time PCR method enables rapid and efficient characterization of acute leukemia in addition to subsequent evaluation of minimal residual diseases.

Original languageEnglish
Pages (from-to)2291-2299
Number of pages9
JournalLeukemia and Lymphoma
Volume43
Issue number12
DOIs
Publication statusPublished - Dec 1 2002
Externally publishedYes

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Real-Time Polymerase Chain Reaction
Leukemia
Cytogenetics
RNA
Bone Marrow
Wilms' Tumor Genes
Cytogenetic Analysis
Residual Neoplasm
Metaphase
Bone Marrow Cells
Tissue Donors
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Osumi, K., Fukui, T., Kiyoi, H., Kasai, M., Kodera, Y., Kudo, K., ... Naoe, T. (2002). Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR. Leukemia and Lymphoma, 43(12), 2291-2299. https://doi.org/10.1080/1042819021000040206

Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR. / Osumi, Kazuoki; Fukui, Takafumi; Kiyoi, Hitoshi; Kasai, Masanobu; Kodera, Yoshihisa; Kudo, Kazuko; Kato, Koji; Matsuyama, Takaharu; Naito, Kazuyuki; Tanimoto, Mitsune; Hirai, Hisamaru; Saito, Hidehiko; Ohno, Ryuzo; Naoe, Tomoki.

In: Leukemia and Lymphoma, Vol. 43, No. 12, 01.12.2002, p. 2291-2299.

Research output: Contribution to journalArticle

Osumi, K, Fukui, T, Kiyoi, H, Kasai, M, Kodera, Y, Kudo, K, Kato, K, Matsuyama, T, Naito, K, Tanimoto, M, Hirai, H, Saito, H, Ohno, R & Naoe, T 2002, 'Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR', Leukemia and Lymphoma, vol. 43, no. 12, pp. 2291-2299. https://doi.org/10.1080/1042819021000040206
Osumi, Kazuoki ; Fukui, Takafumi ; Kiyoi, Hitoshi ; Kasai, Masanobu ; Kodera, Yoshihisa ; Kudo, Kazuko ; Kato, Koji ; Matsuyama, Takaharu ; Naito, Kazuyuki ; Tanimoto, Mitsune ; Hirai, Hisamaru ; Saito, Hidehiko ; Ohno, Ryuzo ; Naoe, Tomoki. / Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR. In: Leukemia and Lymphoma. 2002 ; Vol. 43, No. 12. pp. 2291-2299.
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AU - Kiyoi, Hitoshi

AU - Kasai, Masanobu

AU - Kodera, Yoshihisa

AU - Kudo, Kazuko

AU - Kato, Koji

AU - Matsuyama, Takaharu

AU - Naito, Kazuyuki

AU - Tanimoto, Mitsune

AU - Hirai, Hisamaru

AU - Saito, Hidehiko

AU - Ohno, Ryuzo

AU - Naoe, Tomoki

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