Rapid selection of polyphenol-rich tea trees (Camellia sinensis L.) employing a colorimetric method

Chang Mi Heo, Yong Duck Kim, Taek Keun Oh, Ji Yun Min, Jung Gyu Hwang, Mi Jin Jeong, Hyun Jin Song, Hak Gon Kim, Seon Jeong Sim, Myung Suk Choi, Chandrakant S. Karigar, Hyun Shik Moon, Seung Joo Lee, Yoshiyuki Shinogi

Research output: Contribution to journalArticle

Abstract

An efficient and rapid colorimetric method was established for the selection of tea tree lines rich in polyphenol from 160 tea tree lines. The efficient selection method was made possible by the adoption of the Folin-Ciocalteu (FC) colorimetric method for qualitative detection of the polyphenols. The polyphenol composition varied widely among the randomly sampled 160 tea tree lines. The tea tree lines were classified into three groups on the basis of color intensity obtained after treatment of tea extracts with FC reagent. The polyphenol content of the three groups was quantified and validated by UV spectrophotometric analysis. Among various tea lines, H-23 tea line exhibited the highest amounts of polyphenol that was 2.89-fold higher than those found in low polyphenol producing tea tree (H-9). Hence FC colorimetric method can become a reliable selection method for easy and rapid selection of tea population rich in polyphenol in a tea tree breeding programme.

Original languageEnglish
Pages (from-to)467-471
Number of pages5
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume57
Issue number2
Publication statusPublished - Sep 1 2012

Fingerprint

Camellia sinensis
Polyphenols
Tea
tea
polyphenols
methodology
selection methods
tree breeding
qualitative analysis
rapid methods
Breeding
Color

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Rapid selection of polyphenol-rich tea trees (Camellia sinensis L.) employing a colorimetric method. / Heo, Chang Mi; Kim, Yong Duck; Oh, Taek Keun; Min, Ji Yun; Hwang, Jung Gyu; Jeong, Mi Jin; Song, Hyun Jin; Kim, Hak Gon; Sim, Seon Jeong; Choi, Myung Suk; Karigar, Chandrakant S.; Moon, Hyun Shik; Lee, Seung Joo; Shinogi, Yoshiyuki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 57, No. 2, 01.09.2012, p. 467-471.

Research output: Contribution to journalArticle

Heo, CM, Kim, YD, Oh, TK, Min, JY, Hwang, JG, Jeong, MJ, Song, HJ, Kim, HG, Sim, SJ, Choi, MS, Karigar, CS, Moon, HS, Lee, SJ & Shinogi, Y 2012, 'Rapid selection of polyphenol-rich tea trees (Camellia sinensis L.) employing a colorimetric method', Journal of the Faculty of Agriculture, Kyushu University, vol. 57, no. 2, pp. 467-471.
Heo, Chang Mi ; Kim, Yong Duck ; Oh, Taek Keun ; Min, Ji Yun ; Hwang, Jung Gyu ; Jeong, Mi Jin ; Song, Hyun Jin ; Kim, Hak Gon ; Sim, Seon Jeong ; Choi, Myung Suk ; Karigar, Chandrakant S. ; Moon, Hyun Shik ; Lee, Seung Joo ; Shinogi, Yoshiyuki. / Rapid selection of polyphenol-rich tea trees (Camellia sinensis L.) employing a colorimetric method. In: Journal of the Faculty of Agriculture, Kyushu University. 2012 ; Vol. 57, No. 2. pp. 467-471.
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