Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

M. Nakano; Z. Ding; H. Kasahara; J. Suehiro

doi:10.1109/TRANSDUCERS.2015.7181228

Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

M. Nakano, Z. Ding, H. Kasahara, J. Suehiro

Applied Energy Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

Original language	English
Title of host publication	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1530-1532
Number of pages	3
ISBN (Electronic)	9781479989553
DOIs	https://doi.org/10.1109/TRANSDUCERS.2015.7181228
Publication status	Published - Aug 5 2015
Event	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: Jun 21 2015 → Jun 25 2015

Publication series

Name	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/Territory	United States
City	Anchorage
Period	6/21/15 → 6/25/15

All Science Journal Classification (ASJC) codes

Instrumentation
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2015.7181228

Cite this

Nakano, M., Ding, Z., Kasahara, H., & Suehiro, J. (2015). Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1530-1532). [7181228] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181228

Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. / Nakano, M.; Ding, Z.; Kasahara, H. et al.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1530-1532 7181228 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nakano, M, Ding, Z, Kasahara, H & Suehiro, J 2015, Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181228, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 1530-1532, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 6/21/15. https://doi.org/10.1109/TRANSDUCERS.2015.7181228

Nakano M, Ding Z, Kasahara H, Suehiro J. Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1530-1532. 7181228. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). doi: 10.1109/TRANSDUCERS.2015.7181228

Nakano, M. ; Ding, Z. ; Kasahara, H. et al. / Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1530-1532 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

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abstract = "This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.",

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N2 - This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

AB - This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

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Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

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