Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy

Michihiko Nakano, Z. Ding, H. Kasahara, Junya Suehiro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a new method to determine the size of DNA amplified by polymerase chain reaction (PCR). After PCR, the amplified DNA was attached on dielectric microbeads, then, the DNA labeled microbeads were trapped on a microelectrode by dielectrophoresis (DEP). The size of the DNA was determined by measuring frequency dependent impedance of the trapped microbeads as an impedance spectroscopy. The size of various DNAs, 142, 204, 391, 796 bp, were successfully identified from the impedance spectrograms. This method determines DNA size in 20 min after PCR.

Original languageEnglish
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1530-1532
Number of pages3
ISBN (Electronic)9781479989553
DOIs
Publication statusPublished - Aug 5 2015
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015Jun 25 2015

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period6/21/156/25/15

Fingerprint

Size determination
size determination
polymerase chain reaction
Polymerase chain reaction
beads
DNA
deoxyribonucleic acid
Spectroscopy
impedance
spectroscopy
spectrograms
Microelectrodes
Electrophoresis

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Nakano, M., Ding, Z., Kasahara, H., & Suehiro, J. (2015). Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1530-1532). [7181228] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181228

Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. / Nakano, Michihiko; Ding, Z.; Kasahara, H.; Suehiro, Junya.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1530-1532 7181228 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakano, M, Ding, Z, Kasahara, H & Suehiro, J 2015, Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181228, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 1530-1532, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 6/21/15. https://doi.org/10.1109/TRANSDUCERS.2015.7181228
Nakano M, Ding Z, Kasahara H, Suehiro J. Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1530-1532. 7181228. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). https://doi.org/10.1109/TRANSDUCERS.2015.7181228
Nakano, Michihiko ; Ding, Z. ; Kasahara, H. ; Suehiro, Junya. / Rapid size determination of PCR amplified DNA by beads-based dielectrophoretic impedance spectroscopy. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1530-1532 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).
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