Rapid transcriptional activation and early mRNA turnover of brain natriuretic peptide in cardiocyte hypertrophy

Evidence for brain natriuretic peptide as an "emergency" cardiac hormone against ventricular overload

Osamu Nakagawa, Yoshihiro Ogawa, Hiroshi Itoh, Shin Ichi Suga, Yasato Komatsu, Ichiro Kishimoto, Kazuyoshi Nishino, Takaaki Yoshimasa, Kazuwa Nakao

Research output: Contribution to journalArticle

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Abstract

We previously demonstrated that brain natriuretic peptide (BNP) is a cardiac hormone mainly produced in the ventricle, while the major production site of atrial natriuretic peptide (ANP) is the atrium. To assess the pathophysiological role of BNP in ventricular overload, we have examined the gene expression of BNP, in comparison with that of ANP, in a model of cardiac hypertrophy using cultured neonatal rat ventricular cardiocytes. During cardiocyte hypertrophy evoked by endothelin-1, phenylephrine, or PMA, the steady state level of BNP mRNA increased as rapidly as the "immediate-early" induction of the c-fos gene expression, and reached a maximal level within 1 h. Actinomycin D, a transcriptional inhibitor, completely diminished the response, while the translational blockade with cycloheximide did not inhibit it. In contrast, ANP mRNA began to increase 3 h after the stimulation, and accumulated during cardiocyte hypertrophy. The BNP secretion from ventricular cardiocytes was also stimulated more rapidly than the ANP secretion. Furthermore, the turnover of BNP mRNA was significantly faster than that of ANP mRNA, being consistent with the existence of AUUUA motif in the 3′-untranslated region of BNP mRNA. These results demonstrate that the gene expression of BNP is distinctly regulated from that of ANP at transcriptional and posttranscriptional levels, and indicate that the characteristics of the BNP gene expression are suitable for its possible role as an "emergency" cardiac hormone against ventricular overload.

Original languageEnglish
Pages (from-to)1280-1287
Number of pages8
JournalJournal of Clinical Investigation
Volume96
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

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Brain Natriuretic Peptide
Hypertrophy
Transcriptional Activation
Emergencies
Atrial Natriuretic Factor
Hormones
Messenger RNA
Gene Expression
fos Genes
Cardiomegaly
3' Untranslated Regions
Dactinomycin
Phenylephrine
Endothelin-1
Cycloheximide

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Rapid transcriptional activation and early mRNA turnover of brain natriuretic peptide in cardiocyte hypertrophy : Evidence for brain natriuretic peptide as an "emergency" cardiac hormone against ventricular overload. / Nakagawa, Osamu; Ogawa, Yoshihiro; Itoh, Hiroshi; Suga, Shin Ichi; Komatsu, Yasato; Kishimoto, Ichiro; Nishino, Kazuyoshi; Yoshimasa, Takaaki; Nakao, Kazuwa.

In: Journal of Clinical Investigation, Vol. 96, No. 3, 01.01.1995, p. 1280-1287.

Research output: Contribution to journalArticle

Nakagawa, Osamu ; Ogawa, Yoshihiro ; Itoh, Hiroshi ; Suga, Shin Ichi ; Komatsu, Yasato ; Kishimoto, Ichiro ; Nishino, Kazuyoshi ; Yoshimasa, Takaaki ; Nakao, Kazuwa. / Rapid transcriptional activation and early mRNA turnover of brain natriuretic peptide in cardiocyte hypertrophy : Evidence for brain natriuretic peptide as an "emergency" cardiac hormone against ventricular overload. In: Journal of Clinical Investigation. 1995 ; Vol. 96, No. 3. pp. 1280-1287.
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