Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya, Takahiro Yano, Sanetaka Shirahata, Junko Watanabe, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

Original languageEnglish
Pages (from-to)341-344
Number of pages4
JournalBioscience, biotechnology, and biochemistry
Volume59
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

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Recombinant Proteins
Amplification
Interleukin-6
Plasmids
Cells
Cell Line
ras Genes
Genes
Productivity
Cytomegalovirus
Transfection

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines. / Teruya, Kiichiro; Yano, Takahiro; Shirahata, Sanetaka; Watanabe, Junko; Osada, Kazuhiro; Tachibana, Hirofumi; Murakami, Hiroki; Ohashi, Hideya; Kim, Eun Ho.

In: Bioscience, biotechnology, and biochemistry, Vol. 59, No. 2, 01.01.1995, p. 341-344.

Research output: Contribution to journalArticle

Teruya, Kiichiro ; Yano, Takahiro ; Shirahata, Sanetaka ; Watanabe, Junko ; Osada, Kazuhiro ; Tachibana, Hirofumi ; Murakami, Hiroki ; Ohashi, Hideya ; Kim, Eun Ho. / Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines. In: Bioscience, biotechnology, and biochemistry. 1995 ; Vol. 59, No. 2. pp. 341-344.
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