Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya, Takahiro Yano, Sanetaka Shirahata, Junko Watanabe, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

Original languageEnglish
Pages (from-to)341-344
Number of pages4
JournalBioscience, biotechnology, and biochemistry
Volume59
Issue number2
DOIs
Publication statusPublished - 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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