Abstract
To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 341-344 |
| Number of pages | 4 |
| Journal | Bioscience, biotechnology, and biochemistry |
| Volume | 59 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 1995 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
Cite this
Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines. / Teruya, Kiichiro; Yano, Takahiro; Shirahata, Sanetaka; Watanabe, Junko; Osada, Kazuhiro; Tachibana, Hirofumi; Murakami, Hiroki; Ohashi, Hideya; Kim, Eun Ho.
In: Bioscience, biotechnology, and biochemistry, Vol. 59, No. 2, 01.01.1995, p. 341-344.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines
AU - Teruya, Kiichiro
AU - Yano, Takahiro
AU - Shirahata, Sanetaka
AU - Watanabe, Junko
AU - Osada, Kazuhiro
AU - Tachibana, Hirofumi
AU - Murakami, Hiroki
AU - Ohashi, Hideya
AU - Kim, Eun Ho
PY - 1995/1/1
Y1 - 1995/1/1
N2 - To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
AB - To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=85007744209&partnerID=8YFLogxK
U2 - 10.1271/bbb.59.341
DO - 10.1271/bbb.59.341
M3 - Article
C2 - 7766036
AN - SCOPUS:85007744209
VL - 59
SP - 341
EP - 344
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 2
ER -