Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya; Takahiro Yano; Sanetaka Shirahata; Junko Watanabe; Kazuhiro Osada; Hirofumi Tachibana; Hiroki Murakami; Hideya Ohashi; Eun Ho Kim

doi:10.1271/bbb.59.341

Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya, Takahiro Yano, Sanetaka Shirahata, Junko Watanabe, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

Original language	English
Pages (from-to)	341-344
Number of pages	4
Journal	Bioscience, biotechnology, and biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1271/bbb.59.341
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.59.341

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title = "Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines",

abstract = "To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been {\textquoteleft}primed{\textquoteright} by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.",

author = "Kiichiro Teruya and Takahiro Yano and Sanetaka Shirahata and Junko Watanabe and Kazuhiro Osada and Hirofumi Tachibana and Hiroki Murakami and Hideya Ohashi and Kim, {Eun Ho}",

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doi = "10.1271/bbb.59.341",

language = "English",

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T1 - Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

AU - Teruya, Kiichiro

AU - Yano, Takahiro

AU - Shirahata, Sanetaka

AU - Watanabe, Junko

AU - Osada, Kazuhiro

AU - Tachibana, Hirofumi

AU - Murakami, Hiroki

AU - Ohashi, Hideya

AU - Kim, Eun Ho

PY - 1995

Y1 - 1995

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AB - To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

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JO - Bioscience, Biotechnology and Biochemistry

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Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Abstract

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