Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya; Takahiro Yano; Sanetaka Shirahata; Junko Watanabe; Kazuhiro Osada; Hirofumi Tachibana; Hiroki Murakami; Hideya Ohashi; Eun Ho Kim

doi:10.1271/bbb.59.341

Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Kiichiro Teruya, Takahiro Yano, Sanetaka Shirahata, Junko Watanabe, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been ‘primed’ by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.

Original language	English
Pages (from-to)	341-344
Number of pages	4
Journal	Bioscience, biotechnology, and biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1271/bbb.59.341
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.59.341

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Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines. / Teruya, Kiichiro; Yano, Takahiro; Shirahata, Sanetaka; Watanabe, Junko; Osada, Kazuhiro; Tachibana, Hirofumi; Murakami, Hiroki; Ohashi, Hideya; Kim, Eun Ho.

In: Bioscience, biotechnology, and biochemistry, Vol. 59, No. 2, 1995, p. 341-344.

Research output: Contribution to journal › Article › peer-review

Teruya, Kiichiro ; Yano, Takahiro ; Shirahata, Sanetaka ; Watanabe, Junko ; Osada, Kazuhiro ; Tachibana, Hirofumi ; Murakami, Hiroki ; Ohashi, Hideya ; Kim, Eun Ho. / Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines. In: Bioscience, biotechnology, and biochemistry. 1995 ; Vol. 59, No. 2. pp. 341-344.

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Ras Amplification in BHK-21 Cells Produces a Host Cell Line for Further Rapid Establishment of Recombinant Protein Hyper-Producing Cell Lines

Abstract

All Science Journal Classification (ASJC) codes

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