RASSF6 Expression in Adipocytes Is Down-Regulated by Interaction with Macrophages

Yohei Sanada, Takahiro Kumoto, Haruna Suehiro, Fusanori Nishimura, Norihisa Kato, Yutaka Hata, Alexander Sorisky, Noriyuki Yanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Macrophage infiltration into adipose tissue is associated with obesity and the crosstalk between adipocytes and infiltrated macrophages has been investigated as an important pathological phenomenon during adipose tissue inflammation. Here, we sought to identify adipocyte mRNAs that are regulated by interaction with infiltrated macrophages in vivo. An anti-inflammatory vitamin, vitamin B6, suppressed macrophage infiltration into white adipose tissue and altered mRNA expression. We identified >3500 genes whose expression is significantly altered during the development of obesity in db/db mice, and compared them to the adipose tissue mRNA expression profile of mice supplemented with vitamin B6. We identified PTX3 and MMP3 as candidate genes regulated by macrophage infiltration. PTX3 and MMP3 mRNA expression in 3T3-L1 adipocytes was up-regulated by activated RAW264.7 cells and these mRNA levels were positively correlated with macrophage number in adipose tissue in vivo. Next, we screened adipose genes down-regulated by the interaction with macrophages, and isolated RASSF6 (Ras association domain family 6). RASSF6 mRNA in adipocytes was decreased by culture medium conditioned by activated RAW264.7 cells, and RASSF6 mRNA level was negatively correlated with macrophage number in adipose tissue, suggesting that adipocyte RASSF6 mRNA expression is down-regulated by infiltrated macrophages in vivo. Finally, this study also showed that decreased RASSF6 expression up-regulates mRNA expression of several genes, such as CD44 and high mobility group protein HMGA2. These data provide novel insights into the biological significance of interactions between adipocytes and macrophages in adipose tissue during the development of obesity.

Original languageEnglish
Article numbere61931
JournalPLoS One
Volume8
Issue number4
DOIs
Publication statusPublished - Apr 23 2013
Externally publishedYes

Fingerprint

Macrophages
adipocytes
Adipocytes
macrophages
Messenger RNA
adipose tissue
Adipose Tissue
Tissue
Infiltration
Vitamin B 6
obesity
Obesity
Genes
pyridoxine
High Mobility Group Proteins
Gene Expression
gene expression
White Adipose Tissue
white adipose tissue
mice

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sanada, Y., Kumoto, T., Suehiro, H., Nishimura, F., Kato, N., Hata, Y., ... Yanaka, N. (2013). RASSF6 Expression in Adipocytes Is Down-Regulated by Interaction with Macrophages. PLoS One, 8(4), [e61931]. https://doi.org/10.1371/journal.pone.0061931

RASSF6 Expression in Adipocytes Is Down-Regulated by Interaction with Macrophages. / Sanada, Yohei; Kumoto, Takahiro; Suehiro, Haruna; Nishimura, Fusanori; Kato, Norihisa; Hata, Yutaka; Sorisky, Alexander; Yanaka, Noriyuki.

In: PLoS One, Vol. 8, No. 4, e61931, 23.04.2013.

Research output: Contribution to journalArticle

Sanada, Y, Kumoto, T, Suehiro, H, Nishimura, F, Kato, N, Hata, Y, Sorisky, A & Yanaka, N 2013, 'RASSF6 Expression in Adipocytes Is Down-Regulated by Interaction with Macrophages', PLoS One, vol. 8, no. 4, e61931. https://doi.org/10.1371/journal.pone.0061931
Sanada, Yohei ; Kumoto, Takahiro ; Suehiro, Haruna ; Nishimura, Fusanori ; Kato, Norihisa ; Hata, Yutaka ; Sorisky, Alexander ; Yanaka, Noriyuki. / RASSF6 Expression in Adipocytes Is Down-Regulated by Interaction with Macrophages. In: PLoS One. 2013 ; Vol. 8, No. 4.
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