Rat monoclonal antibody specific for MyoD

Akihito Harada, Yasuyuki Ohkawa, Shinpei Ao, Jun Odawara, Seiji Okada, Masayuki Azuma, Yuko Nishiyama, Mako Nakamura, Taro Tachibana

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

Original languageEnglish
Pages (from-to)255-258
Number of pages4
JournalHybridoma
Volume29
Issue number3
DOIs
Publication statusPublished - Jun 1 2010

Fingerprint

Skeletal Muscle
Monoclonal Antibodies
Helix-Loop-Helix Motifs
Myogenic Regulatory Factors
Myoblasts
Regulator Genes
Transcription Factors
Lymph Nodes
Staining and Labeling
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Harada, A., Ohkawa, Y., Ao, S., Odawara, J., Okada, S., Azuma, M., ... Tachibana, T. (2010). Rat monoclonal antibody specific for MyoD. Hybridoma, 29(3), 255-258. https://doi.org/10.1089/hyb.2009.0117

Rat monoclonal antibody specific for MyoD. / Harada, Akihito; Ohkawa, Yasuyuki; Ao, Shinpei; Odawara, Jun; Okada, Seiji; Azuma, Masayuki; Nishiyama, Yuko; Nakamura, Mako; Tachibana, Taro.

In: Hybridoma, Vol. 29, No. 3, 01.06.2010, p. 255-258.

Research output: Contribution to journalArticle

Harada, A, Ohkawa, Y, Ao, S, Odawara, J, Okada, S, Azuma, M, Nishiyama, Y, Nakamura, M & Tachibana, T 2010, 'Rat monoclonal antibody specific for MyoD', Hybridoma, vol. 29, no. 3, pp. 255-258. https://doi.org/10.1089/hyb.2009.0117
Harada, Akihito ; Ohkawa, Yasuyuki ; Ao, Shinpei ; Odawara, Jun ; Okada, Seiji ; Azuma, Masayuki ; Nishiyama, Yuko ; Nakamura, Mako ; Tachibana, Taro. / Rat monoclonal antibody specific for MyoD. In: Hybridoma. 2010 ; Vol. 29, No. 3. pp. 255-258.
@article{169e43cda79647bba052f1df9bb9626d,
title = "Rat monoclonal antibody specific for MyoD",
abstract = "Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.",
author = "Akihito Harada and Yasuyuki Ohkawa and Shinpei Ao and Jun Odawara and Seiji Okada and Masayuki Azuma and Yuko Nishiyama and Mako Nakamura and Taro Tachibana",
year = "2010",
month = "6",
day = "1",
doi = "10.1089/hyb.2009.0117",
language = "English",
volume = "29",
pages = "255--258",
journal = "Monoclonal Antibodies in Immunodiagnosis and Immunotherapy",
issn = "2167-9436",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Rat monoclonal antibody specific for MyoD

AU - Harada, Akihito

AU - Ohkawa, Yasuyuki

AU - Ao, Shinpei

AU - Odawara, Jun

AU - Okada, Seiji

AU - Azuma, Masayuki

AU - Nishiyama, Yuko

AU - Nakamura, Mako

AU - Tachibana, Taro

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

AB - Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

UR - http://www.scopus.com/inward/record.url?scp=77954187370&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954187370&partnerID=8YFLogxK

U2 - 10.1089/hyb.2009.0117

DO - 10.1089/hyb.2009.0117

M3 - Article

VL - 29

SP - 255

EP - 258

JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

SN - 2167-9436

IS - 3

ER -