Rational design of a protein-based molecular device consisting of blue fluorescent protein and zinc protoporphyrin IX incorporated into a cytochrome b562 scaffold

Shuji Takeda, Noriho Kamiya, Teruyuki Nagamune

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To make a single molecular photo-device, it is essential to control the exact orientation of two types of proteins. We made a chimeric protein in which cytochrome b562 was linked to the N-terminus of enhanced green fluorescent protein, cytb562-EGFP. Within cytb562-EGFP, the excitation energy of EGFP was transferred to the cytochrome b562 cofactor fixed proximally to EGFP. Cytb562-EGFP was engineered so that iron protoporphyrin IX was substituted by zinc protoporphyrin IX to make it a suitable cofactor for photo-induced electron transfer. The photosensitizer pigment was optimized and the EGFP was replaced by a blue fluorescent mutant that gave 15% higher energy transfer efficiency.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalBiotechnology Letters
Volume26
Issue number2
DOIs
Publication statusPublished - Jan 2004

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Cytochromes
Scaffolds (biology)
Scaffolds
Zinc
Proteins
Equipment and Supplies
Photosensitizing Agents
Excitation energy
Energy Transfer
Pigments
Energy transfer
Electrons
Photosensitizers
zinc protoporphyrin
Iron
enhanced green fluorescent protein
iron protoporphyrin IX

All Science Journal Classification (ASJC) codes

  • Biotechnology

Cite this

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