Rational design of a protein-based molecular device consisting of blue fluorescent protein and zinc protoporphyrin IX incorporated into a cytochrome b562 scaffold

Shuji Takeda, Noriho Kamiya, Teruyuki Nagamune

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To make a single molecular photo-device, it is essential to control the exact orientation of two types of proteins. We made a chimeric protein in which cytochrome b562 was linked to the N-terminus of enhanced green fluorescent protein, cytb562-EGFP. Within cytb562-EGFP, the excitation energy of EGFP was transferred to the cytochrome b562 cofactor fixed proximally to EGFP. Cytb562-EGFP was engineered so that iron protoporphyrin IX was substituted by zinc protoporphyrin IX to make it a suitable cofactor for photo-induced electron transfer. The photosensitizer pigment was optimized and the EGFP was replaced by a blue fluorescent mutant that gave 15% higher energy transfer efficiency.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalBiotechnology Letters
Volume26
Issue number2
DOIs
Publication statusPublished - Jan 2004

All Science Journal Classification (ASJC) codes

  • Biotechnology

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