TY - JOUR
T1 - Rational optimization of the DSL ligase ribozyme with GNRA/receptor interacting modules
AU - Ishikawa, Junya
AU - Matsumura, Shigeyoshi
AU - Jaeger, Luc
AU - Inoue, Tan
AU - Furuta, Hiroyuki
AU - Ikawa, Yoshiya
N1 - Funding Information:
This research was partly supported by Grant-in-Aids on Innovative Areas “ Emergence in Chemistry ” [No. 21111518 to Y.I.], for Exploratory Research [No. 19657071 to Y.I.], and the Global COE program, “ Science for Future Molecular Systems ” [to H.F.] from The Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), and also by a grant from the National Institutes of Health [ R01-GM079604 to L.J.]. L.J., H.F., and Y.I. also thank to a joint program between Kyushu University Global COE (Science for Future Molecular Systems) and the California NanoSystems Institute (CNSI) for supporting this research.
PY - 2009/10/15
Y1 - 2009/10/15
N2 - The DSL ribozyme is a class of artificial ligase ribozymes with a highly modular architecture, which catalyzes template-directed RNA ligation on a helical substrate module that can be either covalently connected (cis-DSL) or physically separated (trans-DSL) from the catalytic module. Substrate recognition by the catalytic module is promoted by one or two sets of GNRA/receptor interactions acting as clamps in the cis or trans configurations, respectively. In this study, we have rationally designed and analyzed the catalytic and self-assembly properties of several trans-DSL ribozymes with different sets of natural and artificial GNRA-receptor clamps. Two variants newly designed in this study showed significantly enhanced catalytic properties with respect of the original trans-DSL construct. While this work allows dissection of the turnover and catalytic properties of the trans-DSL ribozyme, it also emphasizes the remarkable modularity of RNA tertiary structure for nano-construction of complex functions.
AB - The DSL ribozyme is a class of artificial ligase ribozymes with a highly modular architecture, which catalyzes template-directed RNA ligation on a helical substrate module that can be either covalently connected (cis-DSL) or physically separated (trans-DSL) from the catalytic module. Substrate recognition by the catalytic module is promoted by one or two sets of GNRA/receptor interactions acting as clamps in the cis or trans configurations, respectively. In this study, we have rationally designed and analyzed the catalytic and self-assembly properties of several trans-DSL ribozymes with different sets of natural and artificial GNRA-receptor clamps. Two variants newly designed in this study showed significantly enhanced catalytic properties with respect of the original trans-DSL construct. While this work allows dissection of the turnover and catalytic properties of the trans-DSL ribozyme, it also emphasizes the remarkable modularity of RNA tertiary structure for nano-construction of complex functions.
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U2 - 10.1016/j.abb.2009.08.020
DO - 10.1016/j.abb.2009.08.020
M3 - Article
C2 - 19728985
AN - SCOPUS:70349753459
VL - 490
SP - 163
EP - 170
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 2
ER -