RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation

Hyun Soo Cho, Shinya Hayami, Gouji Toyokawa, Kazuhiro Maejima, Yuka Yamane, Takehiro Suzuki, Naoshi Dohmae, Masaharu Kogure, Daechun Kang, David E. Neal, Bruce A.J. Ponder, Hiroki Yamaue, Yusuke Nakamura, Ryuji Hamamoto

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

It is well known that RB functions are regulated by posttranslational modifications such as phosphorylation and acetylation, but the significance of lysine methylation on RB has not been fully elucidated. Our expression analysis of SMYD2 by quantitative real-time polymerase chain reaction showed that expression levels of SMYD2 are significantly elevated in human bladder carcinomas compared with nonneoplastic bladder tissues (P <.0001), and its expression levels in tumor tissues were much higher than those of any other normal tissues. SMYD2 knockdown resulted in the suppression of cancer cell growth, and cell cycle analysis indicated that SMYD2 might play a crucial role in the G1/S transition. According to an in vitro methyltransferase assay, we found that SMYD2 methylates RB1 protein, and liquid chromatography-tandem mass spectrometry analysis revealed lysine 810 of RB1 to be methylated by SMYD2. Importantly, this methylation enhanced Ser 807/811 phosphorylation of RB1 both in vitro and in vivo. Furthermore, we demonstrated that methylated RB1 accelerates E2F transcriptional activity and promotes cell cycle progression. SMYD2 is an important oncoprotein in various types of cancer, and SMYD2-dependent RB1 methylation at lysine 810 promotes cell cycle progression of cancer cells. Further study may explore SMYD2-dependent RB1 methylation as a potential therapeutic target in human cancer.

Original languageEnglish
Pages (from-to)476-486
Number of pages11
JournalNeoplasia (United States)
Volume14
Issue number6
DOIs
Publication statusPublished - Jan 1 2012

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Methylation
Cell Cycle
Phosphorylation
Lysine
Neoplasms
Urinary Bladder
Oncogene Proteins
Methyltransferases
Post Translational Protein Processing
Acetylation
Tandem Mass Spectrometry
Liquid Chromatography
Real-Time Polymerase Chain Reaction
Carcinoma
Growth
Proteins
In Vitro Techniques
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Cho, H. S., Hayami, S., Toyokawa, G., Maejima, K., Yamane, Y., Suzuki, T., ... Hamamoto, R. (2012). RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation. Neoplasia (United States), 14(6), 476-486. https://doi.org/10.1593/neo.12656

RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation. / Cho, Hyun Soo; Hayami, Shinya; Toyokawa, Gouji; Maejima, Kazuhiro; Yamane, Yuka; Suzuki, Takehiro; Dohmae, Naoshi; Kogure, Masaharu; Kang, Daechun; Neal, David E.; Ponder, Bruce A.J.; Yamaue, Hiroki; Nakamura, Yusuke; Hamamoto, Ryuji.

In: Neoplasia (United States), Vol. 14, No. 6, 01.01.2012, p. 476-486.

Research output: Contribution to journalArticle

Cho, HS, Hayami, S, Toyokawa, G, Maejima, K, Yamane, Y, Suzuki, T, Dohmae, N, Kogure, M, Kang, D, Neal, DE, Ponder, BAJ, Yamaue, H, Nakamura, Y & Hamamoto, R 2012, 'RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation', Neoplasia (United States), vol. 14, no. 6, pp. 476-486. https://doi.org/10.1593/neo.12656
Cho, Hyun Soo ; Hayami, Shinya ; Toyokawa, Gouji ; Maejima, Kazuhiro ; Yamane, Yuka ; Suzuki, Takehiro ; Dohmae, Naoshi ; Kogure, Masaharu ; Kang, Daechun ; Neal, David E. ; Ponder, Bruce A.J. ; Yamaue, Hiroki ; Nakamura, Yusuke ; Hamamoto, Ryuji. / RB1 methylation by SMYD2 enhances cell cycle progression through an increase of RB1 phosphorylation. In: Neoplasia (United States). 2012 ; Vol. 14, No. 6. pp. 476-486.
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