Reactive oxygen species are involved in gibberellin/ abscisic acid signaling in barley aleurone cells

Yushi Ishibashi, Tomoya Tawaratsumida, Koji Kondo, Shinsuke Kasa, Masatsugu Sakamoto, Nozomi Aoki, Shao Hui Zheng, Takashi Yuasa, Mari Iwaya-Inoue

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)


Reactive oxygen species (ROS) act as signal molecules for a variety of processes in plants. However, many questions about the roles of ROS in plants remain to be clarified. Here, we report the role of ROS in gibberellin (GA) and abscisic acid (ABA) signaling in barley (Hordeum vulgare) aleurone cells. The production of hydrogen peroxide (H 2O 2), a type of ROS, was induced by GA in aleurone cells but suppressed by ABA. Furthermore, exogenous H 2O 2 appeared to promote the induction of a-amylases by GA. In contrast, antioxidants suppressed the induction of a-amylases. Therefore, H 2O 2 seems to function in GA and ABA signaling, and in regulation of a-amylase production, in aleurone cells. To identify the target of H 2O 2 in GA and ABA signaling, we analyzed the interrelationships between H 2O 2 and DELLA proteins Slender1 (SLN1), GA-regulated Myb transcription factor (GAmyb), and ABA-responsive protein kinase (PKABA) and their roles in GA and ABA signaling in aleurone cells. In the presence of GA, exogenous H 2O 2 had little effect on the degradation of SLN1, the primary transcriptional repressor mediating GA signaling, but it promoted the production of the mRNA encoding GAMyb, which acts downstream of SLN1 and involves induction of a-amylase mRNA. Additionally, H 2O 2 suppressed the production of PKABA mRNA, which is induced by ABA:PKABA represses the production of GAMyb mRNA. From these observations, we concluded that H 2O 2 released the repression of GAMyb mRNA by PKABA and consequently promoted the production of α-amylase mRNA, thus suggesting that the H 2O 2 generated by GA in aleurone cells is a signal molecule that antagonizes ABA signaling.

Original languageEnglish
Pages (from-to)1705-1714
Number of pages10
JournalPlant physiology
Issue number4
Publication statusPublished - Apr 2012

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science


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