Reactive oxygen species, nitric oxide, and their interactions play different roles in Cupressus lusitanica cell death and phytoalexin biosynthesis

Jian Zhao, Koki Fujita, Kokki Sakai

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

• β-Thujaplicin is a natural troponoid with strong antifungal, antiviral, and anticancer activities. β-Thujaplicin production in yeast elicitor-treated Cupressus lusitanica cell culture and its relationships with reactive oxygen species (ROS) and nitric oxide (NO) production and hypersensitive cell death were investigated. • Superoxide anion radical (O2•-) induced cell death and inhibited β-thujaplicin accumulation, whereas hydrogen peroxide (H2O 2) induced β-thujaplicin accumulation but did not significantly affect cell death. Both elicitor and O2•- induced programmed cell death, which can be blocked by protease inhibitors, protein kinase inhibitors, and Ca2+ chelators. • Elicitor-induced NO generation was nitric oxide synthase (NOS)-dependent. Inhibition of NO generation by NOS inhibitors and NO scavenger partly blocked the elicitor-induced β-thujaplicin accumulation and cell death, and NO donors strongly induced cell death. • Interaction among NO, H2O 2, and O2•- shows that NO production and H2O2 production are interdependent, but NO and O 2•- accumulation were negatively related because of coconsumption of NO and O2•-. NO- and O 2•--induced cell death required each other, and both were required for elicitor-induced cell death. A direct interaction between NO and O2•- was implicated in the production of a potent oxidant peroxynitrite, which might mediate the elicitor-induced cell death.

Original languageEnglish
Pages (from-to)215-229
Number of pages15
JournalNew Phytologist
Volume175
Issue number2
DOIs
Publication statusPublished - Jul 2007

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Fingerprint

Dive into the research topics of 'Reactive oxygen species, nitric oxide, and their interactions play different roles in Cupressus lusitanica cell death and phytoalexin biosynthesis'. Together they form a unique fingerprint.

Cite this