Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor

Takashi Sakamoto; Masa aki Inoue; Akio Ojida; Itaru Hamachi

doi:10.1016/j.bmcl.2009.05.121

Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor

Takashi Sakamoto, Masa aki Inoue, Akio Ojida, Itaru Hamachi

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We developed a new fluorescence assay system for GSK3β-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.

Original language	English
Pages (from-to)	4175-4177
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	19
Issue number	15
DOIs	https://doi.org/10.1016/j.bmcl.2009.05.121
Publication status	Published - Aug 1 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2009.05.121

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AU - Inoue, Masa aki

AU - Ojida, Akio

AU - Hamachi, Itaru

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ER -

Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor

Abstract

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