Real-time fluorescence monitoring of GSK3β-catalyzed phosphoryation by use of a BODIPY-based Zn(II)-Dpa chemosensor

Takashi Sakamoto, Masa aki Inoue, Akio Ojida, Itaru Hamachi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We developed a new fluorescence assay system for GSK3β-catalyzed kinase reaction using the BODIPY-based fluorescent chemosensor. This system exploits the selective sensing property of the chemosensor for a (i, i+4) bis-phosphorylated peptide, which allows us to conveniently detect the phosphorylation reaction with a fluorescence increase in a real-time fashion.

Original languageEnglish
Pages (from-to)4175-4177
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number15
DOIs
Publication statusPublished - Aug 1 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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