Real-time quantitative reverse transcription-polymerase chain reaction for the detection of AML1-MTG8 fusion transcripts in t(8;21)-positive acute myelogenous leukemia

Masaru Kondo, Kazuko Kudo, Hiroshi Kimura, Jun Inaba, Koji Kato, Seiji Kojima, Takaharu Matsuyama, Keizo Horibe

Research output: Contribution to journalArticle

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Abstract

Quantification of AML1-MTG8 fusion transcripts was performed by using real-time reverse transcription-polymerase chain reaction (RT-PCR) and the clinical value of this method was evaluated in t(8;21)-positive acute myelogenous leukemia (AML). A t(8;21)-positive cell line, Kasumi-1, was used for constructing standard curves and the corrected AML1-MTG8 mRNA expression level relative to the expression of the GAPDH housekeeping gene was calculated. Bone marrow samples from 14 patients with t(8;21)-positive AML were sequentially examined. The corrected AML1-MTG8 expression level at diagnosis varied in the range from 0.4 to 2.7 (median, 1.5) among the patients. When samples at 1, 3 and 6 months were examined after diagnosis, the corrected AML1-MTG8 expression level was found to decrease sequentially in all but one. AML1-MTG8 fusion transcripts were also detected in four of eight samples from patients in remission for more than 1 year. In conclusion, real-time RT-PCR can provide a rapid and accurate quantification of AML1-MTG8 fusion transcripts. This system could be useful to reveal the prognostic relevance of minimal residual disease in t(8;21)-positive AML. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)951-956
Number of pages6
JournalLeukemia Research
Volume24
Issue number11
DOIs
Publication statusPublished - Nov 29 2000
Externally publishedYes

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Acute Myeloid Leukemia
Reverse Transcription
Polymerase Chain Reaction
Essential Genes
Residual Neoplasm
Bone Marrow
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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Real-time quantitative reverse transcription-polymerase chain reaction for the detection of AML1-MTG8 fusion transcripts in t(8;21)-positive acute myelogenous leukemia. / Kondo, Masaru; Kudo, Kazuko; Kimura, Hiroshi; Inaba, Jun; Kato, Koji; Kojima, Seiji; Matsuyama, Takaharu; Horibe, Keizo.

In: Leukemia Research, Vol. 24, No. 11, 29.11.2000, p. 951-956.

Research output: Contribution to journalArticle

Kondo, Masaru ; Kudo, Kazuko ; Kimura, Hiroshi ; Inaba, Jun ; Kato, Koji ; Kojima, Seiji ; Matsuyama, Takaharu ; Horibe, Keizo. / Real-time quantitative reverse transcription-polymerase chain reaction for the detection of AML1-MTG8 fusion transcripts in t(8;21)-positive acute myelogenous leukemia. In: Leukemia Research. 2000 ; Vol. 24, No. 11. pp. 951-956.
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