Realization of 240 nanometer resolution of cell positioning by a virtual flow reduction mechanism

Shinya Sakuma, Keisuke Kuroda, Makoto Kaneko, Fumihito Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

This paper presents the real-time precise positioning of a single cell with extremely high resolution. The positioning system is based on the visual feedback control of the syringe pump. The issue for using a syringe is that the flow rate is geometrically amplified in microchannel due to the ratio of cross sectional areas of the syringe and the microchannel. In order to overcome this issue, we introduce the virtual flow reduction mechanism. This mechanism utilizes the elasticity of poly-dimethylpolysiloxane (PDMS) microfluidic chip where the pressure peak is limited but the pressure response decreases with a sufficiently large time constant compared with the sampling time. By using this characteristic, we design and develop the system together with an online vision system. Through experiments, we could confirm that the cell positioning resolution is 240 nm corresponding to 1 pixel of the vision.

Original languageEnglish
Title of host publicationMEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1031-1034
Number of pages4
ISBN (Print)9781479935086
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States
Duration: Jan 26 2014Jan 30 2014

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/26/141/30/14

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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