TY - GEN
T1 - Realization of 240 nanometer resolution of cell positioning by a virtual flow reduction mechanism
AU - Sakuma, Shinya
AU - Kuroda, Keisuke
AU - Kaneko, Makoto
AU - Arai, Fumihito
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - This paper presents the real-time precise positioning of a single cell with extremely high resolution. The positioning system is based on the visual feedback control of the syringe pump. The issue for using a syringe is that the flow rate is geometrically amplified in microchannel due to the ratio of cross sectional areas of the syringe and the microchannel. In order to overcome this issue, we introduce the virtual flow reduction mechanism. This mechanism utilizes the elasticity of poly-dimethylpolysiloxane (PDMS) microfluidic chip where the pressure peak is limited but the pressure response decreases with a sufficiently large time constant compared with the sampling time. By using this characteristic, we design and develop the system together with an online vision system. Through experiments, we could confirm that the cell positioning resolution is 240 nm corresponding to 1 pixel of the vision.
AB - This paper presents the real-time precise positioning of a single cell with extremely high resolution. The positioning system is based on the visual feedback control of the syringe pump. The issue for using a syringe is that the flow rate is geometrically amplified in microchannel due to the ratio of cross sectional areas of the syringe and the microchannel. In order to overcome this issue, we introduce the virtual flow reduction mechanism. This mechanism utilizes the elasticity of poly-dimethylpolysiloxane (PDMS) microfluidic chip where the pressure peak is limited but the pressure response decreases with a sufficiently large time constant compared with the sampling time. By using this characteristic, we design and develop the system together with an online vision system. Through experiments, we could confirm that the cell positioning resolution is 240 nm corresponding to 1 pixel of the vision.
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U2 - 10.1109/MEMSYS.2014.6765820
DO - 10.1109/MEMSYS.2014.6765820
M3 - Conference contribution
AN - SCOPUS:84899006784
SN - 9781479935086
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 1031
EP - 1034
BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Y2 - 26 January 2014 through 30 January 2014
ER -