Reassessment of mapping of the E homeotic gene complex of Bombyx mori by linkage analysis and in situ hybridization with an Antennapedia clone as a probe

Yutaka Banno, Kosaku Sakaida, Takashi Nakamura, Yutaka Kawaguchi, Katsumi Koga, Hiroshi Doira, Kozo Tsuchida

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8 Citations (Scopus)

Abstract

The gene for a recessive mutant named “soft and limp” (gene symbol sol) was shown to be linked with the E homeotic pseudoalleles previously mapped to the distal end of the 6th linkage group. A three-point test involving one of the E genes (Eca) and the F gene localized on the same linkage group indicated that the arrangement of these three gene loci is in the order of sol—Eca—F. Taking the recombination values and correction factors into account, the locus of the sol gene was determined to be -21.1 cM of the 6th linkage group. These results suggest that the 0.0 position of this linkage group should be the sol gene instead of the E loci. This inference was supported by the results of fluorescence in situ hybridization with an RNA probe derived from the B. mori Antennapedia gene closely linked with the E complex loci; the signal was detected at an internal region of a chromosome, not at its distal end.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalJournal of Sericultural Science of Japan
Volume66
Issue number3
DOIs
Publication statusPublished - Jan 1 1997

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

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