Recent advance in single-cell epigenomics

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Abstract

The analysis of gene expression regulation, or the epigenome analysis, at the single-cell level is at the forefront of genomics research. To elucidate the mechanisms that regulate gene expression, chromatin immunoprecipitation has been conventionally used for determining the binding sites of DNA-binding proteins, such as histones and transcription factors. Now several new approaches have been emerged to reveal epigenome states at the single-cell level. Instead of using immunoprecipitation of fragmented chromatin, in situ reactions using cells or nuclei, combining with transposase tagging and other methods, have enabled single-cell analysis. Furthermore, single-cell multiomics techniques to simultaneously profiling transcriptome and open chromatin or histone modification have been developed. These single-cell analyses have the potential to identify different cell types in a cell population and reveal the dynamic changes of gene regulation, although those technologies have not yet reached a level for general application.

Original languageEnglish
Pages (from-to)116-122
Number of pages7
JournalCurrent Opinion in Structural Biology
Volume71
DOIs
Publication statusPublished - Dec 2021

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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