TY - JOUR
T1 - Recent advances in exploiting ionic liquids for biomolecules
T2 - Solubility, stability and applications
AU - Sivapragasam, Magaret
AU - Moniruzzaman, Muhammad
AU - Goto, Masahiro
N1 - Funding Information:
MS thanks the Centre of Research in Ionic Liquids (CORIL), Universiti Teknologi PETRONAS for financial support (Postdoctoral Fellowship) The authors declare no financial or commercial conflict of interest
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) – potentially attractive ”green“ and ”designer“ solvents – rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.
AB - The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) – potentially attractive ”green“ and ”designer“ solvents – rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.
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U2 - 10.1002/biot.201500603
DO - 10.1002/biot.201500603
M3 - Review article
C2 - 27312484
AN - SCOPUS:84979768773
VL - 11
SP - 1000
EP - 1013
JO - Biotechnology Journal
JF - Biotechnology Journal
SN - 1860-6768
IS - 8
ER -