Recent advances in reversed micellar techniques for bioseparation

S. Furusaki, S. Ichikawa, M. Goto

    Research output: Chapter in Book/Report/Conference proceedingChapter

    6 Citations (Scopus)

    Abstract

    Biocompatible systems of reversed micelles are required for the application to the preparation of food additives or medicinal substances. Use of soybean lecithin or phosphatidylcholine as a surfactant, and ethyl oleate, ethyl linoleate or ethyl caproate as a solvent gave satisfactory systems for this purpose. Oleic acid or cholesterol can be used as a cosolvent. Characterization of the micelles using small angle X-ray scattering (SAXS) is presented. Extraction of DNA using reversed micelles of positively charged surfactants is possible. The effect of carbon number of the surfactants was studied, and distearyl quaternary ammonium chloride was most effective. Alcohol was used as a cosolvent and 1-octanol gave the best result. The circular dichroism (CD) spectrum of the extracted DNA was the same as that in an aqueous solution.

    Original languageEnglish
    Title of host publicationProgress in Biotechnology
    PublisherElsevier
    Pages133-136
    Number of pages4
    EditionC
    DOIs
    Publication statusPublished - 2000

    Publication series

    NameProgress in Biotechnology
    NumberC
    Volume16
    ISSN (Print)0921-0423

    All Science Journal Classification (ASJC) codes

    • Biotechnology

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